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| Status |
Public on Sep 18, 2023 |
| Title |
Accessible high-throughput single-cell whole genome sequencing with paired chromatin accessibility |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Other
|
| Summary |
We present a technique that leverages nucleosome disruption methodologies with the widely adopted 10x Genomics ATAC-seq library preparation workflow to produce scWGS profiles for copy number analysis in high throughput. We further demonstarte the ability to leverage indexed tagmentation using off-the-shelf reagents to perform scWGS multiplexing, or a co-assay that produces scWGS+ATAC data fromt he same cells, also witht he ability to multipelx samples.
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| Overall design |
We performed standard 10x scATAC v2 as a control along with our nucleosome-disruption to produce scWGS data. Data for multipelxed scWGS were produced on the same control cell line as well as for the dTag experiments that produce both ATAC and WGS data from the same cells.
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| |
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| Contributor(s) |
Adey AC, Quietsch K |
| Citation(s) |
37918402 |
| |
| Submission date |
Sep 18, 2023 |
| Last update date |
Dec 14, 2023 |
| Contact name |
Andrew Adey |
| E-mail(s) |
adey@ohsu.edu
|
| Organization name |
Oregon Health & Science University
|
| Department |
MMG
|
| Lab |
Adey Lab
|
| Street address |
3181 S.W. Sam Jackson Park Rd, RHH, L103
|
| City |
Portland |
| State/province |
OR |
| ZIP/Postal code |
97239-3098 |
| Country |
USA |
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| Platforms (1) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA1018483 |
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