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| Status |
Public on Sep 20, 2023 |
| Title |
Multi-species atlas resolves an axolotl limb development and regeneration paradox |
| Organism |
Ambystoma mexicanum |
| Experiment type |
Other
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| Summary |
Humans and other tetrapods are considered to require apical-ectodermal-ridge, AER, cells for limb development, and AER-like cells are suggested to be re-formed to initiate limb regeneration. Paradoxically, the presence of AER in the axolotl, the primary regeneration model organism, remains controversial. Here, by leveraging a single-cell transcriptomics-based multi-species atlas, composed of axolotl, human, mouse, chicken, and frog cells, we first established that axolotls contain cells with AER characteristics. Surprisingly, further analyses and spatial transcriptomics revealed that axolotl limbs do not fully re-form AER cells during regeneration. Moreover, the axolotl mesoderm displays part of the AER machinery, revealing a novel program for limb (re)growth. These results clarify the debate about the axolotl AER and the extent to which the limb developmental program is recapitulated during regeneration.
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| Overall design |
Sample collected from a 8.5 cm axolotl amputated in the calcified region of the lower arm after 5 days of regeneration (5 dpa)
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| Contributor(s) |
Aires R, Zhong J, Tsissios G, Skoufa E, Brandt K, Sandoval-Guzmán T, Aztekin C |
| Citation(s) |
37816738 |
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| Submission date |
Sep 14, 2023 |
| Last update date |
Oct 25, 2023 |
| Contact name |
Tatiana Sandoval-Guzmán |
| E-mail(s) |
tatiana.sandoval_guzman@tu-dresden.de
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| Organization name |
Technische Universität Dresden
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| Department |
Department of Internal Medicine III, Center for Healthy Aging, Medical Faculty Carl Gustav Carus
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| Lab |
Vertebrate Tissue Repair and Regeneration
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| Street address |
Fetscherstrasse 74
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| City |
Dresden |
| State/province |
Saxony |
| ZIP/Postal code |
01307 |
| Country |
Germany |
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| Platforms (1) |
| GPL27159 |
Illumina NovaSeq 6000 (Ambystoma mexicanum) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA1017433 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE243225_RAW.tar |
14.1 Mb |
(http)(custom) |
TAR (of CSV, JPG, JSON, MTX, PNG, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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