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| Status |
Public on Sep 05, 2023 |
| Title |
Modeling Radiation-induced Cardiovascular Dysfunction with Human iPSC-Derived Engineered Heart Tissues |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Low-LET radiation can cause cardiovascular dysfunctions at high-dose rates. For example, photons used in thoracic radiotherapy are known to cause acute cardiac tissue damage with elevated serum cardiac Troponin I level and long-term cardiac complications when delivered as fractionated exposures at high-dose rates. However, the effects of continuous low-dose rate radiation exposure on the heart, which simulate the space radiation environment, have not been well-studied. In this study, we aim to model low-LET space radiation-induced cardiovascular dysfunction using human induced pluripotent stem cell (iPSC)-derived engineered heart tissues (EHTs) exposed to protracted γ-ray irradiation. The investigation of pathophysiological changes in this model may provide insights and guide the development of countermeasures. As a proof-of-principle for the application of this model in drug development, we also tested the protective effect of a mitochondrial-specific antioxidant, MitoTempo, on irradiated EHTs.
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| Overall design |
In this study, EHTs were generated from two healthy iPSC lines (#1 and #2) using an established protocol, comprising of 70% cardiomyocytes (CMs), 15% endothelial cells (ECs) and 15% fibroblasts (FBs). After 10 days, the EHTs were exposed to 137Cs γ-rays continuously at a dose-rate of 9.3 mGy/hour for 5 days. Cells were collected for bulk RNA sequecning from two independent experiments.
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| Contributor(s) |
Cao X, Thomas D, Whitcombb L, Wang M, Chatterjee A, Chicco AJ, Weil MM, Wu JC |
| Citation(s) |
38431383 |
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| Submission date |
Aug 31, 2023 |
| Last update date |
Apr 04, 2024 |
| Contact name |
Joseph C. Wu |
| E-mail(s) |
joewu@stanford.edu
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| Organization name |
Stanford university
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| Department |
Stanford Cardiovascular Institute
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| Lab |
Joseph Wu lab
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| Street address |
240 Pasteur Dr
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| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA1011335 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE242108_counts.csv.gz |
663.9 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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