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Status |
Public on Sep 17, 2010 |
Title |
Chromatin enrichment of TRIM24, estrogen receptor and H3K4me2 in estrogen-treated and -untreated MCF7 cells |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
TRIM24 PHD-Bromo domains exhibit preferential binding to unmethylated H3K4 and acetylated H3K27. TRIM24 is a co-activator of estrogen receptor (ER). The results suggest that specific ER-binding sites are depleted of H3K4me2 with estrogen treatment. TRIM24 binds these sites preferentially and facilitates ER-regulated gene expression, cell survival and proliferation.
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Overall design |
ChIP performed on MCF7 cells +/- estrogen with antibodies against ER, TRIM24 and H3K4me2. ChIP assays of ER, co-activator TRIM24 and H3K4me2 were performed with two concentrations of antibody, without and 6h with estrogen treatment of MCF7 cells. Antibody-enriched samples were sequenced two times, and compared to an IgG negative control and Input. Enriched DNA sequenced by Illumina Solexa.
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Contributor(s) |
Barton MC, Aronow BJ, Akdemir KC, Jameson C, Keddache M, Yiu TT |
Citation(s) |
21164480 |
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Submission date |
Sep 16, 2010 |
Last update date |
May 15, 2019 |
Contact name |
Bruce J Aronow |
E-mail(s) |
bruce.aronow@chmcc.org
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Phone |
513-636-4865
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Organization name |
Cincinnati Children's Hospital Medical Center
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Street address |
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City |
Cincinnati |
State/province |
OH |
ZIP/Postal code |
45229 |
Country |
USA |
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Platforms (1) |
GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
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Samples (7)
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Relations |
SRA |
SRP003566 |
BioProject |
PRJNA130083 |