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| Status |
Public on Apr 01, 2024 |
| Title |
Single-cell gene expression profiling of CD4+ T cells from small intestine lamina propria of mixed Bcl3-/- CD45.2+ and WT CD45.1+ bone marrow recipients [Mixed-bone-marrow_10XscRNAseq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Loss of Bcl3 influences the abundance and functionality of regulatory T cells in several organs but especially in the intestine. We used single-cell RNA sequencing (scRNAseq) to determine the influence of Bcl3 on cellular basis in a competitive environment.
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| Overall design |
We sorted all living CD4+ T cells (CD45+, CD3+, CD4+) from small intestine lamina propria of four mixed Bcl3-/- CD45.2+ and WT CD45.1+ bone marrow recipients via fluorescence-activated cell sorting.
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| Contributor(s) |
Köhler A, Ohnmacht C |
| Citation(s) |
38663461 |
| |
| Submission date |
Aug 23, 2023 |
| Last update date |
Jul 02, 2024 |
| Contact name |
Caspar Ohnmacht |
| E-mail(s) |
caspar.ohnmacht@helmholtz-munich.de
|
| Organization name |
Helmholtz Munich
|
| Department |
Institute for Allergy Research
|
| Street address |
Ingolstädter Landstraße 1
|
| City |
Oberschleißheim |
| ZIP/Postal code |
85764 |
| Country |
Germany |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA1008844 |
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