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| Status |
Public on Nov 15, 2023 |
| Title |
Plasmodium falciparum MORC protein modulates gene expression through interaction with heterochromatin |
| Organism |
Plasmodium falciparum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The MORC (microrchidia) family of proteins is highly conserved in all eukaryotic cells and have been shown to play diverse roles by forming protein-protein interactions with immune-responsive proteins, SWI chromatin remodeling complexes, histone deacetylases, and histone tail modifications across metazoans. To dissect the functional roles of MORC in the human malaria parasite, Plasmodium falciparum, we developed a glmS based ribozyme knockdown system to induce PfMORC knockdown upon glucosamine (GlcN) induction. We further conducted a transcriptomic analysis in PfMORC-HA-glmS knockdown parasites at the asexual stage to investigate alterations in global gene expression. Our results indicate an overrepresentation of downregulated genes belonging to the heterochromatin-associated hypervariable gene family proteins. Overall, we found that PfMORC controls the asexual gene expression of the P. falciparum and can be a potential target for malaria disease containment.
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| Overall design |
Investigation of differential gene expression in PfMORC knockdown Plasmodium falciparum transgenic parasite.
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| Web link |
https://elifesciences.org/reviewed-preprints/92201
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| Contributor(s) |
Singh M, Tojal da Silva I, Adderley J, Doerig C, da Silva Garcia C |
| Citation(s) |
39412522 |
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| Submission date |
Aug 21, 2023 |
| Last update date |
Nov 05, 2024 |
| Contact name |
Maneesh Kumar Singh |
| E-mail(s) |
manish.sings@gmail.com
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| Organization name |
University of Sao Paulo
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| Street address |
Av. Prof. Lineu Prestes, 580
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| City |
Sao Paulo |
| State/province |
SP |
| ZIP/Postal code |
05508-000 |
| Country |
Brazil |
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| Platforms (1) |
| GPL33701 |
DNBSEQ-G400 (Plasmodium falciparum) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA1007682 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE241313_PfMORC_gene_TPM.tsv.txt.gz |
323.5 Kb |
(ftp)(http) |
TXT |
| GSE241313_RAW.tar |
1.2 Mb |
(http)(custom) |
TAR (of SF) |
SRA Run Selector |
| Raw data are available in SRA |
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