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| Status |
Public on Aug 31, 2011 |
| Title |
Secreted Factors from Staphylococcus aureus Biofilm and Planktonic Cultures Differentially Impact Human Keratinocytes, in vitro |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Interactions between human keratinocytes and secreted factors from Staphylococcus aureus biofilm and planktonic cultures were investigated using microarray analysis.
Relative to planktonic secreted factors, biofilm secreted factors up regulated cytokine and chemokine genes in keratinocytes. Genes associated with DNA damage and oxidative stress were also induced in keratinocytes treated with secreted factors from S. aureus biofilm.
Here we show that secreted factors from S. aureus planktonic (PCM) and biofilm (BCM) cultures differentially impact several aspects of wound healing processes.
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| Overall design |
Secreted factors from S. aureus biofilm and planktonic cultures with equivalent population sizes were placed in contact with human foreskin keratinocytes for 4 hours. Keratinocytes were grown to ~90% confluency between passages 4-10.
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| Contributor(s) |
James GA, Secor PR, Fleckman P, Olerud JE, Dowd SE, McInnerney K, Stewart PS |
| Citation(s) |
21693040 |
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| Submission date |
Sep 14, 2010 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Kate McInnerney |
| E-mail(s) |
kmcinnerney@montana.edu
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| Phone |
406-994-5666
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| Fax |
406-994-4926
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| URL |
http://cores.montana.edu
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| Organization name |
Montana State University
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| Department |
Microbiology
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| Lab |
Functional Genomics Facility
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| Street address |
109 Lewis Hall
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| City |
Bozeman |
| State/province |
MT |
| ZIP/Postal code |
59717 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (9)
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| GSM593636 |
HFK cells treated with S.aureus planktonic-conditioned media, replicate 1 |
| GSM593637 |
HFK cells treated with S.aureus planktonic-conditioned media, replicate 2 |
| GSM593638 |
HFK cells treated with S.aureus planktonic-conditioned media, replicate 3 |
| GSM593639 |
HFK cells treated with S.aureus biofilm-conditioned media, replicate 1 |
| GSM593640 |
HFK cells treated with S.aureus biofilm-conditioned media, replicate 2 |
| GSM593641 |
HFK cells treated with S.aureus biofilm-conditioned media, replicate 3 |
| GSM593642 |
HFK cells treated with EpiLife® (EPI) keratinocyte growth medium (control), replicate 1. |
| GSM593643 |
HFK cells treated with EpiLife® (EPI) keratinocyte growth medium (control), replicate 2. |
| GSM593644 |
HFK cells treated with EpiLife® (EPI) keratinocyte growth medium (control), replicate 3. |
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| Relations |
| BioProject |
PRJNA130053 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24118_RAW.tar |
18.2 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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