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| Status |
Public on Apr 15, 2024 |
| Title |
SOD1 is a synthetic lethal target in PPM1D-mutant leukemia cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Using CRISPR/Cas9 dropout screening, we identified superoxide dismutase-1 (SOD1) as a genetic dependency specific to PPM1D-mutant leukemia cells. We found that the mutant cells exhibited a compromised response to oxidative stress that can be rescued with SOD1 inhibitors. PPM1D-mutant fcells also exhibit significant genomic instability, highlighting the essential role of SOD1 in safeguarding against oxidative stress and DNA damage.
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| Overall design |
Transcriptomic profiling of RNA-seq data for WT and PPM1D-mutant Cas9-OCI-AML2 cells with or without SOD1-knockout.
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| Web link |
https://elifesciences.org/reviewed-preprints/91611
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| Contributor(s) |
Zhang L, Goodell MA, Chen C |
| Citation(s) |
37693622, 38896450 |
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| Submission date |
Aug 15, 2023 |
| Last update date |
Jul 01, 2024 |
| Contact name |
Margaret Goodell |
| E-mail(s) |
goodell@bcm.edu
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| Phone |
7137921265
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| Organization name |
Baylor College of Medicine
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| Street address |
1 Baylor Plz, Rm N1030
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| City |
HOUSTON |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
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| Samples (12)
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| GSM7711111 |
O2, PPM1D-mutant, EV, D10, rep1 |
| GSM7711112 |
O2, PPM1D-mutant, EV, D10, rep2 |
| GSM7711113 |
O2, PPM1D-mutant, EV, D10, rep3 |
| GSM7711114 |
O2, PPM1D-mutant, SODKO, D10, rep1 |
| GSM7711115 |
O2, PPM1D-mutant, SODKO, D10, rep2 |
| GSM7711116 |
O2, PPM1D-mutant, SODKO, D10, rep3 |
| GSM7711117 |
O2, WT, EV, D10, rep1 |
| GSM7711118 |
O2, WT, EV, D10, rep2 |
| GSM7711119 |
O2, WT, EV, D10, rep3 |
| GSM7711120 |
O2, WT, SODKO, D10, rep1 |
| GSM7711121 |
O2, WT, SODKO, D10, rep2 |
| GSM7711122 |
O2, WT, SODKO, D10, rep3 |
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| Relations |
| BioProject |
PRJNA1005625 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE240874_RAW.tar |
57.5 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
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