|
| Status |
Public on May 10, 2024 |
| Title |
Proteome-scale tagging and functional screening in mammalian cells by ORFtag [ORFtag] |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Determining protein function in a systematic and comprehensive manner is a key goal in modern molecular biology research but is challenging with current approaches. Here, we introduce ORFtag, a versatile method for the massively parallel protein tagging and interrogation of protein function at the proteome scale with minimal effort and expense. We demonstrate the versatility of ORFtag in mouse and human cells with a series of functional screens for transcriptional activators, repressors and post-transcriptional gene regulatory proteins.
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| Overall design |
3 different functional proteome-wide screens were performed using ORFtag in mouse cell lines.
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| Contributor(s) |
Nemčko F, Himmelsbach M, Loubiere V, Yelagandula R, Pagani M, Fasching N, Brennecke J, Elling U, Stark A, Ameres SL |
| Citation(s) |
38969721 |
| |
| Submission date |
Aug 07, 2023 |
| Last update date |
Aug 10, 2024 |
| Contact name |
Alexander Stark |
| E-mail(s) |
stark@starklab.org
|
| Organization name |
The Research Institute of Molecular Pathology (IMP)
|
| Lab |
Stark Lab
|
| Street address |
Campus-Vienna-Biocenter 1
|
| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
| |
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| Platforms (2) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE225972 |
Proteome-scale tagging and functional screening in mammalian cells by ORFtag |
|
| Relations |
| BioProject |
PRJNA1003248 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE240282_mouse_PTGR_screen_sorted_vs_unsorted.txt.gz |
326.6 Kb |
(ftp)(http) |
TXT |
| GSE240282_mouse_PTGR_sorted_rep1_counts_rev_strand.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
| GSE240282_mouse_PTGR_sorted_rep1_counts_same_strand.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
| GSE240282_mouse_PTGR_sorted_rep2_counts_rev_strand.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
| GSE240282_mouse_PTGR_sorted_rep2_counts_same_strand.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
| GSE240282_mouse_PTGR_unsorted_rep1_counts_rev_strand.txt.gz |
3.3 Mb |
(ftp)(http) |
TXT |
| GSE240282_mouse_PTGR_unsorted_rep1_counts_same_strand.txt.gz |
3.3 Mb |
(ftp)(http) |
TXT |
| GSE240282_mouse_PTGR_unsorted_rep2_counts_rev_strand.txt.gz |
1.0 Mb |
(ftp)(http) |
TXT |
| GSE240282_mouse_PTGR_unsorted_rep2_counts_same_strand.txt.gz |
1.0 Mb |
(ftp)(http) |
TXT |
| GSE240282_mouse_activating_screen_sorted_vs_unsorted.txt.gz |
232.8 Kb |
(ftp)(http) |
TXT |
| GSE240282_mouse_activating_sorted_rep1_counts_rev_strand.txt.gz |
347.8 Kb |
(ftp)(http) |
TXT |
| GSE240282_mouse_activating_sorted_rep1_counts_same_strand.txt.gz |
348.5 Kb |
(ftp)(http) |
TXT |
| GSE240282_mouse_activating_sorted_rep2_counts_rev_strand.txt.gz |
260.6 Kb |
(ftp)(http) |
TXT |
| GSE240282_mouse_activating_sorted_rep2_counts_same_strand.txt.gz |
261.1 Kb |
(ftp)(http) |
TXT |
| GSE240282_mouse_activating_unsorted_rep1_counts_rev_strand.txt.gz |
2.6 Mb |
(ftp)(http) |
TXT |
| GSE240282_mouse_activating_unsorted_rep1_counts_same_strand.txt.gz |
2.6 Mb |
(ftp)(http) |
TXT |
| GSE240282_mouse_activating_unsorted_rep2_counts_rev_strand.txt.gz |
2.9 Mb |
(ftp)(http) |
TXT |
| GSE240282_mouse_activating_unsorted_rep2_counts_same_strand.txt.gz |
2.9 Mb |
(ftp)(http) |
TXT |
| GSE240282_mouse_repressor_screen_sorted_vs_unsorted.txt.gz |
222.4 Kb |
(ftp)(http) |
TXT |
| GSE240282_mouse_repressor_sorted_rep1_counts_rev_strand.txt.gz |
362.4 Kb |
(ftp)(http) |
TXT |
| GSE240282_mouse_repressor_sorted_rep1_counts_same_strand.txt.gz |
363.2 Kb |
(ftp)(http) |
TXT |
| GSE240282_mouse_repressor_sorted_rep2_counts_rev_strand.txt.gz |
266.6 Kb |
(ftp)(http) |
TXT |
| GSE240282_mouse_repressor_sorted_rep2_counts_same_strand.txt.gz |
267.0 Kb |
(ftp)(http) |
TXT |
| GSE240282_mouse_repressor_unsorted_rep1_counts_rev_strand.txt.gz |
5.1 Mb |
(ftp)(http) |
TXT |
| GSE240282_mouse_repressor_unsorted_rep1_counts_same_strand.txt.gz |
5.1 Mb |
(ftp)(http) |
TXT |
| GSE240282_mouse_repressor_unsorted_rep2_counts_rev_strand.txt.gz |
5.2 Mb |
(ftp)(http) |
TXT |
| GSE240282_mouse_repressor_unsorted_rep2_counts_same_strand.txt.gz |
5.2 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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