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| Status |
Public on Sep 14, 2023 |
| Title |
Formate supplementation enhances anti-tumor CD8+ T cell fitness and efficacy of PD-1 blockade |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Metabolic support of the response to immune checkpoint blockage is unknown. We used scRNA-seq to determine the response of CD8+ cells to metabolic supplementation with the one carbon donor formate in combination with anti-PD-1
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| Overall design |
Tumor infiltrating CD8+ cells were purified by FACS on day 12 post implantation from B16-OVA tumors after treatment with formate (starting day 9) and antibody (starting day 10). Four treatment groups, control drinking water with isotype control antibody (C.I.), formate drinking water with isotype control antibody (F.I.), control drinking water with anti-PD-1 antibody (C.P.), and formate drinking water with anti-PD-1 antibody (F.P.).
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| Web link |
https://aacrjournals.org/cancerdiscovery/article-abstract/doi/10.1158/2159-8290.CD-22-1301/729147/Formate-supplementation-enhances-anti-tumor-CD8-T?redirectedFrom=fulltext
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| Contributor(s) |
Arlene S, Marcia H, Jared R, Ilaria E |
| Citation(s) |
37728660 |
| |
| Submission date |
Aug 07, 2023 |
| Last update date |
Nov 22, 2023 |
| Contact name |
Osmaan Shahid |
| E-mail(s) |
osmaanshahid@gmail.com
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| Organization name |
Harvard Medical School
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| Department |
Department of Immunology
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| Lab |
Sharpe Lab
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| Street address |
77 Avenue Louis Pasteur
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA1003017 |
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