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| Status |
Public on Dec 29, 2023 |
| Title |
scRNAseq of Cd45+ cells from brains of ApoE-variant Alzheimer's mice sorted by ex-vivo amyloid beta uptake |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here we perform single cell sequencing of immune cells of aged AD mice bearing knock-in of human APOE variants in the murine APOE locus, sorted into high-uptake and low-uptake pools after incubation with fluorescently labeled amyloid beta.
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| Overall design |
We produced single cell suspensions from the hippocampal and cortical regions of 60-week-old 5XFAD-hemizygous mice bearing homozygous knock-in of APOE2, APOE3, or APOE4 in the murine Apoe allele. Cells were incubated with fluorescently labeled amyloid beta before being sorted for Cd45+ and split into a high- and low-uptake pool. Cells were hashed post-sort and sequenced as a single library by 10X 5'v2 single cell sequencing.
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| Web link |
https://www.cell.com/immunity/fulltext/S1074-7613(23)00532-0
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| Contributor(s) |
Millet A |
| Citation(s) |
38159571 |
| |
| Submission date |
Aug 03, 2023 |
| Last update date |
Jan 25, 2024 |
| Contact name |
Alon Millet |
| E-mail(s) |
amillet@rockefeller.edu
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| Organization name |
The Rockefeller University
|
| Lab |
Tavazoie Lab
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| Street address |
1230 York Ave
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
| GSM7679367 |
E2/E3/E4 5XFAD mice, 60 weeks, high and low abeta uptake, scRNAseq |
| GSM7679368 |
E2/E3/E4 5XFAD mice, 60 weeks, high and low abeta uptake, HTO for demuxing |
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| This SubSeries is part of SuperSeries: |
| GSE239999 |
An Exhausted-Like Microglial Population Accumulates in Aged and APOE4 Genotype Alzheimer’s Brains |
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| Relations |
| BioProject |
PRJNA1001859 |
