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| Status |
Public on Mar 04, 2024 |
| Title |
Effect of KDM5 histone demethylase in 3T3-L1 differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The KDM5 histone demethylases regulate histone methylation to modulate chromatin structure and gene expression. Using a KDM5 enzyme inhibitor (KDM5-C70) and transcriptome profiling by RNA-sequencing in 3T3-L1 cells at different states of differentiation, we determined that KDM5 activity influences the induction of genes associated with cell cycle regulation and mitochondrial function.
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| Overall design |
3T3-L1 preadipocytes were treated with 10 µM KDM5-C70 (KDM5 inhibitor) or DMSO control at differentiation Day –2 (100% confluency). Cells were collected for RNA at differentiation Day 0, 1, 2, and 7.
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| Contributor(s) |
Vergnes L, Reue K, Wiese CB |
| Citation(s) |
38425435 |
| |
| Submission date |
Aug 03, 2023 |
| Last update date |
Jun 03, 2024 |
| Contact name |
Karen Reue |
| E-mail(s) |
reuek@g.ucla.edu
|
| Organization name |
University of California, Los Angeles
|
| Street address |
695 Charles E. Young Drive South
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (29)
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| Relations |
| BioProject |
PRJNA1001810 |
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