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| Status |
Public on Oct 18, 2023 |
| Title |
Cytosolic PRRs are required to upregulate interferon gene expression upon EZH2 inhibition with GSK343 in B16-F10 melanoma cells [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
EZH2 is a histone methyltransferase that deposits the repressive histone H3 lysine 27 trimethylation (H3K27me3). This mark has been reported to silence expression of various repetitive elements in the genome. We sought to identify the effect of EZH2 inhibition on coding and non-coding, repetitive RNA expression. We generated a novel mutant mouse line whose ability to detect induced repeat element expression has been abrogated. We report that the mutant splenic B cells fail to upregulate inflammatory gene expression upon EZH2 inhibition, despite comparable upregulation of various repetitive elements with WT. Unlike WT splenic B cells that do not upregulate interferon gene expression upon EZH2 inhibition, B16-F10 melanoma cells activate their expression under the same inhibitor treatment. Cytosolic pattern recognition receptors, RIG-I and Cgas, are required for such response as their deletion abrogates this pathway.
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| |
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| Overall design |
Gene expression profiles of WT and PRR mutant B16-F10 cells upon GSK343 treatment
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| |
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| Contributor(s) |
Dick FA, Kim SJ |
| Citation(s) |
37934086 |
| |
| Submission date |
Aug 02, 2023 |
| Last update date |
Jan 17, 2024 |
| Contact name |
Frederick Andrew Dick |
| E-mail(s) |
fdick@uwo.ca
|
| Organization name |
Western University
|
| Department |
Pathology
|
| Lab |
A4-VRL LRCP
|
| Street address |
790 Commissioners Road E
|
| City |
London |
| State/province |
ON |
| ZIP/Postal code |
N6A 4L6 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE198232 |
Cytosolic pattern recognition receptors respond to EZH2-inhibition induced viral mimicry response that eliminates splenic B cells |
|
| Relations |
| BioProject |
PRJNA1001317 |
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