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Series GSE23852 Query DataSets for GSE23852
Status Public on Jun 03, 2011
Title Activator protein-2γ is a critical determinant of estrogen receptor interactome formation and gene transcription in breast cancer [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Estrogen receptor α (ERα) is key player in the progression of breast cancer. ERα binds to DNA and mediates long-range chromatin interactions throughout the genome, but the underlying mechanism in this process is unclear. Here, we show that AP-2 motifs are highly enriched in the ERα binding sites (ERBS) identified from the recent ChIA-PET of ERα. More importantly, we demonstrate that AP-2γ (also known as TFAP2C), a member of the AP-2 family which has been implicated in breast cancer oncogenesis, is recruited to chromatin in a ligand-independent manner and co-localized with ERα binding events. Furthermore, pertubation of AP-2γ expression disrupts ERα DNA binding, long-range chromatin interactions, and gene transcription. Using ChIP-seq, we show that AP-2γ and ERα binding occurs in close proximity on a genome-wide scale. The majority of these shared genomic regions are also occupied by the pioneer factor, FoxA1. AP-2γ is required for efficient FoxA1 binding and vice versa. Finally, we show that most ERBS associated with long-range chromatin interactions are co-localized with both AP-2γ and FoxA1. Together, our results suggest AP-2γ is an essential factor in ERα-mediated transcription, primarily working together with FoxA1 to facilitate ERα binding and long-range chromatin interactions.
Overall design Genome-wide binding analysis of AP-2γ and FoxA1 in MCF-7 with and without E2 (estradiol) stimulation using ChIP-Seq.
Contributor(s) Tan SK, Lin Z, Chang CW, Cheung E
Citation(s) 21572391
Submission date Aug 27, 2010
Last update date May 15, 2019
Contact name SI KEE TAN
Organization name Genomic Institute of Singapore
Department Cancer biology and Pharmacology
Street address 60 Biopolis Street #02-01 Genome
City Singapore
ZIP/Postal code Singapore 138672
Country Singapore
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (4)
GSM588927 AP-2γ ChIP sequencing in ETOH (ethanol) treated MCF-7 cells
GSM588928 AP-2γ ChIP sequencing in E2 (estradiol) treated MCF-7 cells
GSM588929 FoxA1 ChIP sequencing in ETOH (ethanol) treated MCF-7 cells
This SubSeries is part of SuperSeries:
GSE26741 Activator protein-2γ is a critical determinant of estrogen receptor interactome formation and gene transcription in breast cancer
SRA SRP003811
BioProject PRJNA142709

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Supplementary file Size Download File type/resource
GSE23852_RAW.tar 650.0 Mb (http)(custom) TAR (of BED, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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