|
| Status |
Public on Feb 19, 2024 |
| Title |
HyperTRIBE assay of VSV-GFP-infected HEK293T cells overexpressing ADAR1-CD, PTENα-N(WT)-ADAR1-CD, or PTENα-N(6R/D)-ADAR1-CD fusion protein |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To explore the binding sites of PTENα on viral or host RNAs, we performed HyperTRIBE assay of HEK293T cells overexpressing ADAR1-CD, PTENα-N(WT)-ADAR1-CD, or PTENα-N(6R/D)-ADAR1-CD fusion protein under VSV-GFP (green fluorescent protein-expressing vesicular stomatitis virus) infection.
|
| |
|
| Overall design |
Five 10cm2 dishes of HEK293T cells at 70% confluency were transfected with 10μg of pCCL-EF1a-CMV-NGFR plasmid encoding ADAR1-CD, PTENα-N(WT)-ADAR1-CD, or PTENα-N(6R/D)-ADAR1-CD fusion protein per dish. At 24 hours after transfection, NGFR-positive cells were sorted and infected with VSV-GFP (MOI = 0.01). 6 hours later, cells were collected and total RNA was separated using TRIzol reagent.
|
| |
|
| Contributor(s) |
Yang Z, Zhang X, Lu D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 21, 2023 |
| Last update date |
Feb 20, 2024 |
| Contact name |
Zeliang Yang |
| E-mail(s) |
zeliangy@bjmu.edu.cn
|
| Organization name |
Peking University Health Science Center
|
| Street address |
No.38 Xueyuan Road,Haidian
|
| City |
Beijing |
| ZIP/Postal code |
100191 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (3) |
|
| Relations |
| BioProject |
PRJNA997220 |
