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| Status |
Public on Nov 13, 2023 |
| Title |
The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [Th2 ChIP] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The transcription factor Gata3 is essential both for CD4+ T cell development in the thymus and for the differentiation of Th2 cells, which mediate responses against extracellular parasites and contribute to asthma and allergies. Here we report that the transcription factor Zfp281, in part redundantly with its paralog Zfp148, cooperates with Gata3 to promote CD4+ T cell development and Th2 cell differentiation. Disruption of both factors in T cells resulted in reduced numbers of spleen CD4+ T cells. Additionally, we show that Zfp281 interacts with Gata3, and that Zfp281 binds to Th2 cytokine loci in Th2 cells. Finally, we found that Zfp148 and Zfp281 promote Th2 cytokine expression. During airway inflammation, Zfp148/281-deficient CD4+ T cells failed to express Th2 cytokines, despite normal expression of Gata3. Altogether our data identify Zfp148 and Zfp281 as essential for enabling Gata3 functions in both CD4+ T cell development and Th2 function.
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| Overall design |
CD4+ T cells were activated with IL-4, anti-IFNg, and anti-IL12 (Th2 conditions) for 5 days, then processed for ChIP-Seq
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| Contributor(s) |
Chopp LB, Bosselut R |
| Citation(s) |
37948511 |
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| Submission date |
Jul 20, 2023 |
| Last update date |
Nov 14, 2023 |
| Contact name |
Remy Bosselut |
| E-mail(s) |
bosselur@helix.nih.gov
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| Phone |
240-760-6866
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| Organization name |
National Cancer Institute, National Institute of Health
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| Department |
Laboratory of Immune Cell Biology
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| Lab |
Bosselut
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| Street address |
9000 Rockville Pike, Building 37
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE207572 |
Zfp281 and Zfp148 control CD4+ T cell thymic development and Th2 functions |
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| Relations |
| BioProject |
PRJNA996959 |
