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Series GSE23769 Query DataSets for GSE23769
Status Public on Aug 24, 2010
Title Embryo BAG neurons tiling array
Project modENCODE
Organism Caenorhabditis elegans
Experiment type Expression profiling by genome tiling array
Summary modENCODE_submission_2499
This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648
Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf

Keywords: Transcript tiling array analysis
 
Overall design EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: MT17370 (engineered, target gene gcy-33 tagged by GFP); Tissue: BAG neurons (embryonic); Developmental Stage: Mixed stage of embryos 20dC; Genotype: nIs242[gcy-33::GFP];lin-15(n765); Sex: Hermaphrodite; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Strain MT17370 (engineered, target gene gcy-33 tagged by GFP); temperature 25; Developmental Stage Mixed stage of embryos 20dC; Tissue BAG neurons (embryonic)
Web link http://www.ncbi.nlm.nih.gov/geo/info/ENCODE.html
http://www.modencode.org
 
Contributor(s) Waterston B, Miller D, Gerstein M, Wilson M, Argiwal A, Reinke V
Citation(s) 21173231, 21177967
BioProject PRJNA168961
Submission date Aug 24, 2010
Last update date Apr 30, 2013
Contact name DCC modENCODE
E-mail help@modencode.org
Phone 416-673-8579
Organization name Ontario Institute for Cancer Research
Lab modENCODE DCC
Street address MaRS Centre, South Tower, 101 College Street, Suite 800
City Toronto
State/province Ontario
ZIP/Postal code M5G 0A3
Country Canada
 
Platforms (1)
GPL5634 [Ce25b_MR] Affymetrix C. elegans Tiling 1.0R Array
Samples (3)
GSM586712 embryo_BAG_neurons_tiling_array extraction1_array1
GSM586713 embryo_BAG_neurons_tiling_array extraction2_array1
GSM586714 embryo_BAG_neurons_tiling_array extraction3_array1

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23769_RAW.tar 199.9 Mb (http)(custom) TAR (of BEDGRAPH, CEL)
GSE23769_emb-BAG_smooth_log2_winsize110.bedgraph.gz 29.9 Mb (ftp)(http) BEDGRAPH
GSE23769_emb-BAG_tars_maxgap30_minrun100_thresh9.36.gff3.gz 963.0 Kb (ftp)(http) GFF3
Raw data provided as supplementary file
Processed data provided as supplementary file
Processed data is available on Series record

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