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Series GSE237426 Query DataSets for GSE237426
Status Public on Jul 21, 2023
Title EIF4A1 is essential in B cells by promoting activation-induced translation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary EIF4A1 and cofactors EIF4B and EIF4H have been well characterised in cancers, including B cell malignancies, for their ability to promote the translation of oncogenes with structured 5’ untranslated regions but very little is known of their roles in non-malignant cells. Using mouse models to delete Eif4a1, Eif4b or Eif4h in B cells we show that EIF4A1, but not EIF4B or EIF4H, is essential for B cell development and the germinal centre response. Following activation, EIF4A1 facilitates an increased rate of protein synthesis, MYC expression and expression of cell cycle regulators. However, EIF4A1-deficient cells remain viable whereas Hippuristanol treatment induces cell death.
 
Overall design RNA-seq libraries were prepared from B cells following 24h activation, with four replicates from 8-12-week-old control and Eif4a1 KO mice. Proteomics analysis was also performed from the same samples; data are available using the accession provided in the associated manuscript. Note that replicate 1 for both control and KO were excluded from downstream analysis due to low cell viability following activation.
 
Contributor(s) Screen M, Matheson LS, Turner M
Citation(s) 38011999
Submission date Jul 14, 2023
Last update date Dec 14, 2023
Contact name Louise Matheson
E-mail(s) louise.matheson@babraham.ac.uk
Organization name The Babraham Institute
Department Laboratory of Lymphocyte Signalling and Development
Street address Babraham Research Campus
City Cambridge
ZIP/Postal code CB22 3AT
Country United Kingdom
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM7612588 Ctrl 1
GSM7612589 Ctrl 2
GSM7612591 Ctrl 3
Relations
BioProject PRJNA994938

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE237426_Raw_counts_all.txt.gz 711.7 Kb (ftp)(http) TXT
GSE237426_TPM_all.txt.gz 1.2 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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