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Series GSE237426 Query DataSets for GSE237426
Status Public on Jul 21, 2023
Title EIF4A1 is essential in B cells by promoting activation-induced translation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary EIF4A1 and cofactors EIF4B and EIF4H have been well characterised in cancers, including B cell malignancies, for their ability to promote the translation of oncogenes with structured 5’ untranslated regions but very little is known of their roles in non-malignant cells. Using mouse models to delete Eif4a1, Eif4b or Eif4h in B cells we show that EIF4A1, but not EIF4B or EIF4H, is essential for B cell development and the germinal centre response. Following activation, EIF4A1 facilitates an increased rate of protein synthesis, MYC expression and expression of cell cycle regulators. However, EIF4A1-deficient cells remain viable whereas Hippuristanol treatment induces cell death.
Overall design RNA-seq libraries were prepared from B cells following 24h activation, with four replicates from 8-12-week-old control and Eif4a1 KO mice. Proteomics analysis was also performed from the same samples; data are available using the accession provided in the associated manuscript. Note that replicate 1 for both control and KO were excluded from downstream analysis due to low cell viability following activation.
Contributor(s) Screen M, Matheson LS, Turner M
Citation(s) 38011999
Submission date Jul 14, 2023
Last update date Dec 14, 2023
Contact name Louise Matheson
Organization name The Babraham Institute
Department Laboratory of Lymphocyte Signalling and Development
Street address Babraham Research Campus
City Cambridge
ZIP/Postal code CB22 3AT
Country United Kingdom
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM7612588 Ctrl 1
GSM7612589 Ctrl 2
GSM7612591 Ctrl 3
BioProject PRJNA994938

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Supplementary file Size Download File type/resource
GSE237426_Raw_counts_all.txt.gz 711.7 Kb (ftp)(http) TXT
GSE237426_TPM_all.txt.gz 1.2 Mb (ftp)(http) TXT
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