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Status |
Public on Oct 05, 2023 |
Title |
A single-cell atlas of pig gastrulation as a resource for comparative embryology |
Organism |
Sus scrofa |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The bilaminar disc of early pig embryos closely mirrors that of humans making it a powerful model for studying gastrulation. Given the difficulties obtaining embryos in non-rodents, early cell-fate decisions during mammalian gastrulation remain ill-understood. Here we present a single-cell transcriptomic atlas of pig gastrulation and early organogenesis. We uncover the dynamics of cell fate emergence during pig peri-gastrulation and reveal conserved and species-specific transcriptional programs across different mammals. Combined with investigations in embryos and embryonic stem cells, we elucidate the spatial, molecular, and temporal events during definitive endoderm (DE) formation. We show that early FOXA2+ epiblast progenitors become DE without undergoing epithelial-to-mesenchymal transition, contrasting later emerging FOXA2/TBXT+ anterior primitive streak, which form node/notochord progenitors. We demonstrate that DE fate is driven by hypoblast-derived NODAL signalling, which is extinguished upon DE differentiation. These findings highlight the interplay between temporal and topological signalling during early cell fate decisions during mammalian gastrulation.
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Overall design |
Single-cell RNA sequencing of 62 pig embryos at 8 different time points ranging from the beginning of gastrulation (E11.5) through to the 12 somite stage (E15) using the 10X chromium platform.
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Web link |
https://www.nature.com/articles/s41467-024-49407-6
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Contributor(s) |
Simpson LA, Strange A, Klisch D, Kraunsoe S, Azami T, Goszczynski D, Le T, Planells B, Holmes N, Sang F, Henson S, Loose M, Nichols J, Alberio R |
Citation(s) |
38890321 |
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Submission date |
Jul 07, 2023 |
Last update date |
Jul 15, 2024 |
Contact name |
Ramiro Alberio |
E-mail(s) |
ramiro.alberio@nottingham.ac.uk
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Phone |
+447473159996
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Organization name |
University of Nottingham
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Department |
School of Biosciences
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Lab |
Room Room B234 South Lab
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Street address |
Sutton Bonington Campus
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City |
Sutton Bonington |
State/province |
Leicestershire |
ZIP/Postal code |
LE12 5RD |
Country |
United Kingdom |
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Platforms (1) |
GPL20983 |
Illumina NextSeq 500 (Sus scrofa) |
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Samples (24)
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Relations |
BioProject |
PRJNA992403 |
Supplementary file |
Size |
Download |
File type/resource |
GSE236766_All_samples_metadata.csv.gz |
3.1 Mb |
(ftp)(http) |
CSV |
GSE236766_All_samples_raw_counts.csv.gz |
265.6 Mb |
(ftp)(http) |
CSV |
GSE236766_RAW.tar |
1.7 Gb |
(http)(custom) |
TAR (of TAR) |
Raw data not provided for this record |
Processed data provided as supplementary file |
Processed data are available on Series record |
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