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| Status |
Public on Aug 01, 2023 |
| Title |
Spatiotemporal hepatocyte heterogeneity impacts in vivo gene engineering [Visium Spatial Transcriptomics] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
The liver is a central organ for physiology and metabolism, with hepatocytes being the main cell type responsible for most of its functions. Several previous studies investigated the cell types involved in tissue homeostasis and regeneration, however the mechanisms underlying post-natal liver growth and establishment of the mature hepatocyte phenotypes remain to be fully understood. Moreover, genetic modification of hepatocytes is emerging as a promising therapeutic approach, particularly for genetic diseases of the coagulation system and hepatic metabolism. Here, we investigate liver tissue dynamics in mice during post-natal growth and turnover in adulthood, by spatial transcriptomics, clonal analysis, and lineage tracing. We observe progressive establishment of metabolic zonation of hepatocytes and that only a fraction of hepatocytes proliferates in the newborn liver, generating most of the adult tissue. We assess the impact of these changes on both in vivo liver gene transfer and gene editing. We show that the age at treatment affects both the efficiency and lobule distribution of lentiviral vector-mediated gene transfer, and that preferential targeting of the more proliferating hepatocytes allows expansion of the genetically modified liver area. Overall, our findings provide new insights into the spatio-temporal dynamics of the liver during post-natal growth and hepatocyte heterogeneity, with broad implications for liver biology and therapeutic applications.
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| Overall design |
Spatial transcriptomics of 17 mouse livers, from newborn, juvenile or adult mice, either LV-transduced, 3 days post LV, or age-matched untransduced mice.
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| Contributor(s) |
Starinieri F, Milani M, Beretta S, Merelli I, Cantore A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jul 03, 2023 |
| Last update date |
May 03, 2024 |
| Contact name |
Stefano Beretta |
| E-mail(s) |
beretta.stefano1@hsr.it
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| Organization name |
San Raffaele Telethon Institute for Gene Therapy
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| Street address |
Via Olgettina, 60
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| City |
Milan |
| ZIP/Postal code |
20132 |
| Country |
Italy |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (23)
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| This SubSeries is part of SuperSeries: |
| GSE236425 |
Spatiotemporal hepatocyte heterogeneity impacts in vivo gene engineering |
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| Relations |
| BioProject |
PRJNA990790 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE236424_RAW.tar |
581.1 Mb |
(http)(custom) |
TAR (of CSV, JPG, JSON, MTX, PNG, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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