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Series GSE23553 Query DataSets for GSE23553
Status Public on Oct 03, 2011
Title Gene expression changes with induction of in-vitro platinum-resistance in ovarian cancer cell lines.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We treated 8 human ovarian cancer cell lines with cisplatin in treatment/recovery cycles to induce in-vitro resistance to the drug. Microarrays measured gene expression at baseline and after each dose schedule (after recovery).
Overall design OVCA cells lines (T8, OVCAR5, OV2008, IGROV1, C13, A2780S, A2780CP, and A2008) were used in the study. Cells were subject to sequential treatment with increasing doses of cis-diammine-dichloroplatinum (cisplatin, CIS), using three dosing schedules resulting in a total of 144 treatment/expansion cycles. Treatment schedules A, B and C included three treatments with 1, 2 and 3μg/ml CIS respectively, followed by three treatments with 3, 4 and 5μg/ml, respectively. Each CIS-treatment was followed by a cell recovery/expansion phase. Both CIS-resistance and genome-wide expression changes were measured serially in each cell line at baseline and after 3 and 6 CIS-treatment/expansion cycles. CIS-resistance was quantified using CellTiter-96, MTS proliferation assays (Fisher Scientific) and genome-wide expression was performed using Affymetrix Human U133 Plus 2.0 GeneChips as previously described.
Contributor(s) Marchion D, Lancaster J, Eschrich S
Citation(s) 21849418
Submission date Aug 10, 2010
Last update date Mar 25, 2019
Contact name Steven Eschrich
Organization name Moffitt Cancer Center
Department Biostatistics & Bioinformatics
Street address 12902 Magnolia Drive
City Tampa
State/province FL
ZIP/Postal code 33612
Country USA
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (56)
GSM577767 A2008
GSM577768 A2008_1.3.3
GSM577769 A2008_2.3.3
This SubSeries is part of SuperSeries:
GSE23603 Gene expression in ovarian cancer
BioProject PRJNA133363

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23553_RAW.tar 270.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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