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Series GSE23505 Query DataSets for GSE23505
Status Public on Aug 31, 2010
Title Enhanced Pathogenicity of Th17 cells Generated in the Absence of Transforming Growth Factor-β Signaling
Organism Mus musculus
Experiment type Expression profiling by array
Summary CD4+ T cells that selectively produce interleukin (IL)-17, are critical for host defense and autoimmunity1-4. Crucial for T helper17 (Th17) cells in vivo5,6, IL-23 has been thought to be incapable of driving initial differentiation. Rather, IL-6 and transforming growth factor (TGF)-β1 have been argued to be the factors responsible for initiating specification7-10. Herein, we show that Th17 differentiation occurs in the absence of TGF-β signaling. Neither IL-6 nor IL-23 alone efficiently generated Th17 cells; however, these cytokines in combination with IL-1β effectively induced IL-17 production in naïve precursors, independently of TGF-β. Epigenetic modification of the Il17a/Il17f and Rorc promoters proceeded without TGF-β1, allowing the generation of cells that co-expressed Rorγt and T-bet. T-bet+Rorγt+ Th17 cells are generated in vivo during experimental allergic encephalomyelitis (EAE), and adoptively transferred Th17 cells generated with IL-23 in the absence of TGF-β1 were more pathogenic in this experimental disease. These data suggest a new model for Th17 differentiation. Consistent with genetic data linking the IL23R with autoimmunity, our findings re-emphasize the role of IL-23 and therefore have important implications for the development of new therapies.
 
Overall design Mouse T helper 17 cell differentiation with or without TGFB
 
Contributor(s) Ghoreschi K, Laurence A, Yang X, Tato CM, McGeachy MJ, Konkel J, Ramos HL, Wei L, Davidson T, Bouladoux N, Grainger J, Chen Q, Kanno Y, Watford WT, Sun H, Eberl G, Shevach E, Belkaid Y, Cua DJ, Chen W, O’Shea JJ
Citation(s) 20962846
Submission date Aug 09, 2010
Last update date Feb 11, 2019
Contact name Lai Wei
E-mail(s) weil2@mail.nih.gov
Phone 3014961480
Organization name NIH/NEI/NCCAM
Street address 10 Center Dr. Room 2B47
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (10)
GSM575743 No_Cytokine_1
GSM575744 No_Cytokine_2
GSM575745 IL6_IL1_1
Relations
BioProject PRJNA131077

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23505_RAW.tar 60.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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