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| Status |
Public on Dec 31, 2011 |
| Title |
Expression data from osteocytes in virgin,lactating and post lactation mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We have previously shown that during lactation, osteocytes directly remodel their perilacunar and pericanalicular matrix, thereby mobilizing calcium and contributing to maternal bone loss. To identify genes potentially responsible for perilacunar remodeling, microarray analysis was performed on osteocytes from CD-1 virgin and lactating mice and mice sacrificed on day 7 post weaning.
By comparing how gene expression changes in osteocytes among virgin, lactation and post lactation, the goal is to identify the genes osteocytes use to remodel their perilacunar matrix
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| Overall design |
To identify genes responsible for perilacunar remodeling, microarray analysis was performed on tibiae from CD-1 virgin and lactating mice and mice sacrificed on day 7 post weaning. Periosteum and bone marrow were removed from the tibiae and the bone fragments underwent sequential collagenase/trypsin digestion and were examined histologically to ensure removal of all surface cells and confirm osteocyte enrichment prior to RNA extraction. Microarray Affy M430 arrays, n=3 per group were performed and Genepattern and Bioconductor were used to analyse the microdata.
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| Contributor(s) |
Qing H, Bonewald LF |
| Citation(s) |
22308018 |
| |
| Submission date |
Aug 06, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Lynda F Bonewald |
| E-mail(s) |
bonewaldl@umkc.edu
|
| Phone |
816 235-2068
|
| Organization name |
School of dentistry University of Missouri-Kansas City
|
| Department |
Department of Oral Biology
|
| Street address |
650 E 25th Street
|
| City |
KANSAS CITY |
| State/province |
MO |
| ZIP/Postal code |
64108 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA131005 |
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