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| Status |
Public on Nov 08, 2023 |
| Title |
Ex Vivo Expansion Potential of Murine Hematopoietic Stem Cells: A Rare Property Only Partially Predicted by Phenotype |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
The scarcity of hematopoietic stem cells (HSCs) restricts their use in both clinical settings and experimental research. Here, we examined a recently developed method for expanding rigorously purified murine HSCs ex vivo. Input HSCs displayed varying potential for ex vivo self-renewal, with alternative outcomes revealed by single cell multimodal RNA- and ATAC-seq profiling. While most HSC progeny offered only transient in vivo reconstitution, these cells efficiently rescued mice from lethal myeloablation. The amplification of functional HSC activity allowed for long-term multilineage engraftment in unconditioned hosts that somewhat surprisingly associated with a return of HSCs to quiescence. Thereby, our findings identify several key considerations for ex vivo HSC expansion, with major implications also for assessment of normal HSC activity.
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| Overall design |
To gain a deeper understanding of the cells generated in ex vivo HSC cultures, we collected cells from the entire culture or EPCR+ cells and isolated nuclei for single cell multiome sequencing
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| Web link |
https://elifesciences.org/reviewed-preprints/91826v1
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| |
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| Contributor(s) |
Zhang Q, Olofzon R, Konturek-Ciesla A, Yuan O, Bryder D |
| Citation(s) |
38446538 |
| |
| Submission date |
Jun 14, 2023 |
| Last update date |
Mar 20, 2024 |
| Contact name |
Qinyu Zhang |
| E-mail(s) |
qinyu.zhang@med.lu.se
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| Organization name |
Lund University
|
| Department |
Department of Molecular Hematology
|
| Lab |
David Bryder lab
|
| Street address |
Box 188
|
| City |
Lund |
| ZIP/Postal code |
22100 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA983719 |
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