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| Status |
Public on Jul 03, 2023 |
| Title |
ChIP-seq in Azin2-/- and wt adipocyte progenitors |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
AZIN2 regulates adipogenesis and acetyl-coA levels in adipocyte progenitors. We investigated here how AZIN2 deficiency affects histone acetylation in adipocyte progenitors.
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| Overall design |
Adipocyte progenitors were isolated from subcutaneous adipose tissue of Azin2-/- and wild-type mice and cultured for 1 week 4.5 g/L glucose Glutamax–DMEM with 10% FBS and 1% penicillin/streptomycin at 37°C and 5% CO2. Then, they were cultured in medium containing 5 µg/ml insulin , 0.5 mM 3-isobutyl-1-methylxantine, 1 µM dexamethasone and 1 µM rosiglitazone. After 2 days, they were switched to medium containing 5 µg/ml insulin and cultured for another 5 days. Medium was changed every 2 days. ChIP was performed using the EZ-Magna ChIP HiSens (Millipore) following manufacturer's instructions. An antibody against H3K27Ac (Millipore 17- 683) was used. DNA was isolated and sequenced.
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| Contributor(s) |
Alexaki V, Sinha A |
| Citation missing |
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| |
| Submission date |
Jun 14, 2023 |
| Last update date |
Jul 04, 2023 |
| Contact name |
Ismini Vasileia Alexaki |
| E-mail(s) |
VasileiaIsmini.Alexaki@ukdd.de
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| Organization name |
IKL , Carl Gustav Carus Universitätsklinikum, TU Dresden
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| Street address |
Fetscherstraße 74
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| City |
Dresden |
| State/province |
Saxony |
| ZIP/Postal code |
01307 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE235012 |
Azin2-dependent transcriptional and epigenetic regulation in adipose tissue and adipocyte progenitors |
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| Relations |
| BioProject |
PRJNA983689 |
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