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Series GSE234861 Query DataSets for GSE234861
Status Public on Nov 15, 2023
Title Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice [MEDIP-seq]
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary Background: Neonicotinoids, a widely used class of insecticide that used is agriculture to control insect’s population, are serious public concern. Objectives: In this study, we examined the capacity of a neonicotinoid, thiacloprid (thia) to induce transgenerational effects in the male mice. Methods: The pregnant outbred Swiss female mice were exposed to thia at embryonic days E6.5 to E15.5 using “0”, and “6” mg/kg/day doses. The genome-wide analysis of DNA methylation analysis showed that differentially methylated regions (DMRs) are located at the promoters and enhancers of many important developmental genes. Notably, DMRs were largely overlapping the germ cell reprograming responsive gene (GRRs) and super-enhancer elements found to be active in ES cells, testis or brain.
 
Overall design MEDIP-seq of spermatozoa from first, second, and third generation adults.
Methylated DNA precipitation DNA-sequencing (MEDIP-seq) for methylated DNA in mouse spermatozoa was performed using 6 ug of DNA using EpiMark® Methylated DNA Enrichment Kit (Qiagen, E2600S) according to protocol provided by manufacture.
 
Contributor(s) Smagulova F
Citation(s) 37973188
Submission date Jun 13, 2023
Last update date Dec 06, 2023
Contact name Fatima SMAGULOVA
E-mail(s) fatima.smagulova@inserm.fr
Phone +3323233662
Organization name Irset - U1085
Lab Chromatin, Epigenetics, Environment
Street address 9 av. Prof. Léon Bernard
City Rennes
ZIP/Postal code 35000
Country France
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (21)
GSM7475971 F1 control, DNA, MBD2-Fc, mouse spermatozoa 1
GSM7475972 F1 control, DNA, MBD2-Fc, mouse spermatozoa 2
GSM7475973 F1 control, DNA, MBD2-Fc, mouse spermatozoa 3
This SubSeries is part of SuperSeries:
GSE235215 Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice
Relations
BioProject PRJNA983361

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Supplementary file Size Download File type/resource
GSE234861_RAW.tar 1.9 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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