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| Status |
Public on Apr 01, 2024 |
| Title |
Single nuclei RNAseq of the periosteal response to bone fracture in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Bone regeneration is a highly efficient process allowing scarless healing after injury. The periosteum, the outer layer of bones, is a critical source of skeletal stem/progenitor cells (SSPCs), as well as immune, endothelial and neural cells during bone repair. In our study, we generated a single-nuclei atlas of the murine periosteal response to bone fracture. We generated single nuclei datasets from uninjured periosteum and from injured periosteum and hematoma/fracture callus at days 5 and 7 post-injury from wild-type mice.
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| Overall design |
Nuclei were extracted from uninjured periosteum, or injured periosteum and hematoma/callus at day 5 and 7 post-fracture. Nuclei were FACS-sorted and processed for single nuclei transcriptomics.
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| Contributor(s) |
Perrin S, Colnot C |
| Citation |
https://doi.org/10.7554/eLife.92519.1
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| Submission date |
Jun 08, 2023 |
| Last update date |
Apr 02, 2024 |
| Contact name |
Céline Colnot |
| E-mail(s) |
celine.colnot@inserm.fr
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| Organization name |
INSERM U955
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| Lab |
Colnot Lab
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| Street address |
8 rue du Général Sarrail
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| City |
Créteil |
| ZIP/Postal code |
94000 |
| Country |
France |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| GSM7468834 |
SC14 - Periosteum, uninjured tibia |
| GSM7468835 |
SC24 - Periosteum, uninjured tibia |
| GSM7468836 |
SC22 - Periosteum and hematoma, tibia 3 days post-fracture |
| GSM7468837 |
SC25 - Periosteum and hematoma, tibia 3 days post-fracture |
| GSM7468838 |
SC12 - Periosteum and hematoma, tibia 5 days post-fracture |
| GSM7468839 |
SC17 - Periosteum and hematoma, tibia 7 days post-fracture |
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| Relations |
| BioProject |
PRJNA981459 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE234451_RAW.tar |
73.3 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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