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Series GSE234354 Query DataSets for GSE234354
Status Public on Sep 06, 2023
Title A molecular staging model for accurately dating the endometrial biopsy [RNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Natural variability in menstrual cycle length, coupled with rapid changes in endometrial gene expression, makes it difficult to accurately define and compare different stages of the endometrial cycle. We have developed and validated a novel method for precisely determining endometrial cycle stage based on global gene expression. Our ‘molecular staging model’ reveals significant and remarkably synchronized daily changes in expression for over 3,400 endometrial genes throughout the cycle, with the most dramatic changes occurring during the secretory phase.
 
Overall design Total RNA from endometrial biopsy was obtained from women across the menstrual cycle. Cycle stages were evaluated using histopathology. Menstrual cycle stage definitions, assuming a standardized 28 day cycle, and numbers of samples in each group were as follows: Stage 1 = menstrual (days 1–4), 2 = early proliferative (days 5–7), 3 = mid proliferative (days 8–11), 4 = late proliferative (days 12–15, includes ‘interval’), 5 = early secretory (days 16–19, or post ovulation days 2-5), 6 = mid secretory (days 20–23, or post ovulation days 6-9), 7 = late secretory (days 24–28, or post ovulation days 10-14).
 
Contributor(s) Rogers PA, Chung J, Teh WT, Mortlock S, Fung JN, Montgomery GW
Citation(s) 37798294
Submission date Jun 07, 2023
Last update date Oct 11, 2023
Contact name Peter Rogers
Organization name University of Melbourne
Department Department of Obstetrics and Gynaecology
Street address Level 7, University Department, Royal Women's Hospital, Crn Grattan Street and Flemington Rd
City Parkville
State/province VIC
ZIP/Postal code 3052
Country Australia
 
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (295)
GSM7465596 endometrial biopsy, X210000
GSM7465597 endometrial biopsy, X210013
GSM7465598 endometrial biopsy, X210013_2
This SubSeries is part of SuperSeries:
GSE234434 A molecular staging model for accurately dating the endometrial biopsy
Relations
BioProject PRJNA981113

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE234354_gene_count_matrix.txt.gz 13.6 Mb (ftp)(http) TXT
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Processed data are available on Series record
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