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| Status |
Public on Oct 08, 2024 |
| Title |
Single-cell transcriptome profiling implicates the psychological stress-induced disruption of spermatogenesis |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Male infertility has become a global problem. And psychological stress has been proven to be an important cause of male infertility. In this work, we constructed a terrified-sound stress model in SD rats and performed single-cell RNA sequencing of the testicular tissues from the post-stressed and normal rats. Through the comparative analysis of the gene expression patterns of the 11,744 rat testicular cells, we revealed the potential mechanism by which terrified-sound induced psychological stress affects spermatogenesis and ultimately leads to reproductive impairment in male rats. This will provide a theoretical basis for the study of psychological stress on male reproductive impairments for other animals and humans, and further find the therapeutic targets for male infertility caused by psychological stress.
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| Overall design |
We constructed a terrified-sound stress model in SD rats and performed single-cell RNA sequencing of the testicular tissues from 3 post-stressed rats and 3 normal rats.
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| Contributor(s) |
Li R, Du Y, Li K, Xiong X, Zhang L, Guo C, Gao S, Yao Y, Xu Y, Yang J |
| Citation(s) |
38439912 |
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| Submission date |
Jun 01, 2023 |
| Last update date |
Oct 09, 2024 |
| Contact name |
Yungang Xu |
| E-mail(s) |
yungang.xu@xjtu.edu.cn
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| Organization name |
Xi'an Jiaotong University
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| Street address |
Xi'an, Shaanxi Province
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| City |
Xi'an, Shaanxi Province |
| State/province |
Shaanxi |
| ZIP/Postal code |
710049 |
| Country |
China |
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| Platforms (1) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA978632 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE233950_RAW.tar |
245.7 Mb |
(http)(custom) |
TAR (of TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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