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Series GSE233743

Query DataSets for GSE233743

Status

Public on May 30, 2023

Title

Comparison of the therapeutic effects between stem cells and exosomes in primary ovarian insufficiency: As promising as cells but different persistency and dosage.

Organism

Experiment type

Expression profiling by high throughput sequencing

Summary

Primary ovarian insufficiency (POI) refers to the loss of ovarian function under the age of 40 and results in amenorrhea and infertility. Our previous studies have shown that transplantation of mesenchymal stem cells (MSCs) and MSC-derived exosomes in chemotherapy-induced POI mouse ovaries can reverse the POI and eventually achieve pregnancy. Based on our recent studies, MSC-derived exosomes have almost equal therapeutic potentials as transplanted MSCs. However, it is still unclear whether exosomes can completely replace MSCs in POI treatment. In this study, we induced POI in C57/BL6 mice by chemotherapy (CXT) using a standard protocol. We compared the RNA expression pattern in ovarian tissue after MSC or exosome treatment. our data suggest that a minimal 10-fold increase, and ideally 100-fold increase, in the exosome concentration is required to generate more analogous results to MSC treatment.

Overall design

To find difference between exosome treatment and MSC treatment (10,000 cells), we injected same amount of exosome particle produced from 10,000 cells(1-fold), higher amounts of exosome particles (10-fold and 100-fold) into the POI model and analyzed ovarian follicles. Next, we compared the RNA expression pattern in ovarian tissue. Expriment 1 (Exp1) is MSC treated set, experiment 2 (Exp2) is UC-exosome treated set, experiment 3(Exp3) is BM-exosome treated set.

Web link

https://pubmed.ncbi.nlm.nih.gov/37340468/

Contributor(s)

Park H, Al-Hendy A

Citation(s)

Submission date

May 30, 2023

Last update date

Aug 29, 2023

Contact name

Hang-soo Park

E-mail(s)

hspark06@bsd.uchicago.edu

Phone

3126149339

Organization name

University of Chicago

Street address

5821 S Drexel Ave

City

Chicago

State/province

IL

ZIP/Postal code

60637

Country

USA

Platforms (1)

Illumina NovaSeq 6000 (Mus musculus)

Samples (38)

More...

GSM7433778	Exp1 Control mouse ovary #1
GSM7433779	Exp1 Control mouse ovary #2
GSM7433780	Exp1 Control mouse ovary #3

Relations

BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE233743_Exp1_D.vs.T1_deg_down_fdr_0.05_fc_1.5.csv.gz	116.7 Kb	(ftp)(http)	CSV
GSE233743_Exp1_D.vs.T1_deg_up_fdr_0.05_fc_1.5.csv.gz	96.8 Kb	(ftp)(http)	CSV
GSE233743_Exp1_D.vs.T1_top_hits.csv.gz	1.0 Mb	(ftp)(http)	CSV
GSE233743_Exp1_D.vs.T2_deg_down_fdr_0.05_fc_1.5.csv.gz	7.4 Kb	(ftp)(http)	CSV
GSE233743_Exp1_D.vs.T2_deg_up_fdr_0.05_fc_1.5.csv.gz	2.6 Kb	(ftp)(http)	CSV
GSE233743_Exp1_D.vs.T2_top_hits.csv.gz	1009.8 Kb	(ftp)(http)	CSV
GSE233743_Exp1_H.vs.D_deg_down_fdr_0.05_fc_1.5.csv.gz	98.4 Kb	(ftp)(http)	CSV
GSE233743_Exp1_H.vs.D_deg_up_fdr_0.05_fc_1.5.csv.gz	126.0 Kb	(ftp)(http)	CSV
GSE233743_Exp1_H.vs.D_gsea_H.csv.gz	2.1 Kb	(ftp)(http)	CSV
GSE233743_Exp1_H.vs.D_top_hits.csv.gz	1.0 Mb	(ftp)(http)	CSV
GSE233743_Exp1_T1.vs.D_deg_down_fdr_0.05_fc_1.5.csv.gz	97.3 Kb	(ftp)(http)	CSV
GSE233743_Exp1_T1.vs.D_deg_up_fdr_0.05_fc_1.5.csv.gz	116.2 Kb	(ftp)(http)	CSV
GSE233743_Exp1_T1.vs.D_gsea_H.csv.gz	3.1 Kb	(ftp)(http)	CSV
GSE233743_Exp1_T1.vs.D_top_hits.csv.gz	1.0 Mb	(ftp)(http)	CSV
GSE233743_Exp1_T1.vs.T2_deg_down_fdr_0.05_fc_1.5.csv.gz	25.6 Kb	(ftp)(http)	CSV
GSE233743_Exp1_T1.vs.T2_deg_up_fdr_0.05_fc_1.5.csv.gz	22.0 Kb	(ftp)(http)	CSV
GSE233743_Exp1_T1.vs.T2_gsea_H.csv.gz	3.8 Kb	(ftp)(http)	CSV
GSE233743_Exp1_T1.vs.T2_top_hits.csv.gz	1013.3 Kb	(ftp)(http)	CSV
GSE233743_Exp1_T2.vs.D_deg_down_fdr_0.05_fc_1.5.csv.gz	2.6 Kb	(ftp)(http)	CSV
GSE233743_Exp1_T2.vs.D_deg_up_fdr_0.05_fc_1.5.csv.gz	7.4 Kb	(ftp)(http)	CSV
GSE233743_Exp1_T2.vs.D_gsea_H.csv.gz	2.7 Kb	(ftp)(http)	CSV
GSE233743_Exp1_T2.vs.D_top_hits.csv.gz	1009.5 Kb	(ftp)(http)	CSV
GSE233743_Exp1_counts.pc.salmon.limma.voom.rds.gz	1.7 Mb	(ftp)(http)	RDS
GSE233743_Exp1_fullheatmap_Exp2.tiff.gz	27.4 Kb	(ftp)(http)	TIFF
GSE233743_Exp1_fullheatmap_onlydiffpairs_Exp2.tiff.gz	28.1 Kb	(ftp)(http)	TIFF
GSE233743_Exp1_gene_counts_salmon.csv.gz	688.0 Kb	(ftp)(http)	CSV
GSE233743_Exp1_log2_cpm_filtered_norm.txt.gz	1.2 Mb	(ftp)(http)	TXT
GSE233743_Exp1_process_count_data_salmon_834_exp2.html.gz	5.5 Mb	(ftp)(http)	HTML
GSE233743_Exp1_sample_list.docx.gz	10.8 Kb	(ftp)(http)	DOCX
GSE233743_Exp1_vplot_D.vs.T1.tiff.gz	33.3 Kb	(ftp)(http)	TIFF
GSE233743_Exp1_vplot_D.vs.T2.tiff.gz	28.6 Kb	(ftp)(http)	TIFF
GSE233743_Exp1_vplot_H.vs.D.tiff.gz	29.4 Kb	(ftp)(http)	TIFF
GSE233743_Exp1_vplot_T1.vs.D.tiff.gz	33.4 Kb	(ftp)(http)	TIFF
GSE233743_Exp1_vplot_T1.vs.T2.tiff.gz	28.6 Kb	(ftp)(http)	TIFF
GSE233743_Exp1_vplot_T2.vs.D.tiff.gz	28.4 Kb	(ftp)(http)	TIFF
GSE233743_Exp2_H.vs.D_deg_down_fdr_0.05_fc_1.5.csv.gz	17.2 Kb	(ftp)(http)	CSV
GSE233743_Exp2_H.vs.D_deg_up_fdr_0.05_fc_1.5.csv.gz	36.4 Kb	(ftp)(http)	CSV
GSE233743_Exp2_H.vs.D_gsea_H.csv.gz	3.3 Kb	(ftp)(http)	CSV
GSE233743_Exp2_H.vs.D_top_hits.csv.gz	1.0 Mb	(ftp)(http)	CSV
GSE233743_Exp2_T1.vs.D_deg_down_fdr_0.05_fc_1.5.csv.gz	672 b	(ftp)(http)	CSV
GSE233743_Exp2_T1.vs.D_deg_up_fdr_0.05_fc_1.5.csv.gz	145 b	(ftp)(http)	CSV
GSE233743_Exp2_T1.vs.D_gsea_H.csv.gz	3.4 Kb	(ftp)(http)	CSV
GSE233743_Exp2_T1.vs.D_top_hits.csv.gz	1014.2 Kb	(ftp)(http)	CSV
GSE233743_Exp2_T2.vs.D_deg_down_fdr_0.05_fc_1.5.csv.gz	4.0 Kb	(ftp)(http)	CSV
GSE233743_Exp2_T2.vs.D_deg_up_fdr_0.05_fc_1.5.csv.gz	788 b	(ftp)(http)	CSV
GSE233743_Exp2_T2.vs.D_gsea_H.csv.gz	2.2 Kb	(ftp)(http)	CSV
GSE233743_Exp2_T2.vs.D_top_hits.csv.gz	1014.4 Kb	(ftp)(http)	CSV
GSE233743_Exp2_T3.vs.D_deg_down_fdr_0.05_fc_1.5.csv.gz	133.5 Kb	(ftp)(http)	CSV
GSE233743_Exp2_T3.vs.D_deg_up_fdr_0.05_fc_1.5.csv.gz	146.6 Kb	(ftp)(http)	CSV
GSE233743_Exp2_T3.vs.D_gsea_H.csv.gz	3.9 Kb	(ftp)(http)	CSV
GSE233743_Exp2_T3.vs.D_top_hits.csv.gz	1.0 Mb	(ftp)(http)	CSV
GSE233743_Exp2_counts.pc.salmon.limma.voom.rds.gz	2.1 Mb	(ftp)(http)	RDS
GSE233743_Exp2_fullheatmap_Exp1.tiff.gz	26.0 Kb	(ftp)(http)	TIFF
GSE233743_Exp2_fullheatmap_onlydiffpairs_Exp1.tiff.gz	26.4 Kb	(ftp)(http)	TIFF
GSE233743_Exp2_gene_counts_salmon.csv.gz	915.0 Kb	(ftp)(http)	CSV
GSE233743_Exp2_log2_cpm_filtered_norm.txt.gz	1.8 Mb	(ftp)(http)	TXT
GSE233743_Exp2_process_count_data_salmon_834_exp1.html.gz	5.4 Mb	(ftp)(http)	HTML
GSE233743_Exp2_sample_list.docx.gz	11.1 Kb	(ftp)(http)	DOCX
GSE233743_Exp2_vplot_H.vs.D.tiff.gz	29.7 Kb	(ftp)(http)	TIFF
GSE233743_Exp2_vplot_T1.vs.D.tiff.gz	27.3 Kb	(ftp)(http)	TIFF
GSE233743_Exp2_vplot_T2.vs.D.tiff.gz	27.5 Kb	(ftp)(http)	TIFF
GSE233743_Exp2_vplot_T3.vs.D.tiff.gz	30.9 Kb	(ftp)(http)	TIFF
GSE233743_Exp3_Disease.vs.Control_HALLMARK_E2F_TARGETS_gsea_H.csv.gz	2.8 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Disease.vs.Control_HALLMARK_EPITHELIAL_MESENCHYMAL_TRANSITION_gsea_H.csv.gz	1.6 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Disease.vs.Control_HALLMARK_MYC_TARGETS_V1_gsea_H.csv.gz	2.3 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Disease.vs.Control_HALLMARK_TNFA_SIGNALING_VIA_NFKB_gsea_H.csv.gz	2.3 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Disease.vs.Control_HALLMARK_UV_RESPONSE_DN_gsea_H.csv.gz	1.5 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Disease.vs.Control_deg_down_fdr_0.05_fc_1.5.csv.gz	13.6 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Disease.vs.Control_deg_up_fdr_0.05_fc_1.5.csv.gz	13.8 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Disease.vs.Control_gsea_H.csv.gz	3.4 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Disease.vs.Control_top_hits.csv.gz	960.9 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Trt1.vs.Disease_deg_down_fdr_0.05_fc_1.5.csv.gz	156 b	(ftp)(http)	CSV
GSE233743_Exp3_Trt1.vs.Disease_deg_up_fdr_0.05_fc_1.5.csv.gz	293 b	(ftp)(http)	CSV
GSE233743_Exp3_Trt1.vs.Disease_top_hits.csv.gz	952.0 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Trt2.vs.Disease_deg_down_fdr_0.05_fc_1.5.csv.gz	156 b	(ftp)(http)	CSV
GSE233743_Exp3_Trt2.vs.Disease_deg_up_fdr_0.05_fc_1.5.csv.gz	295 b	(ftp)(http)	CSV
GSE233743_Exp3_Trt2.vs.Disease_top_hits.csv.gz	932.4 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Trt3.vs.Disease_HALLMARK_DNA_REPAIR_gsea_H.csv.gz	722 b	(ftp)(http)	CSV
GSE233743_Exp3_Trt3.vs.Disease_HALLMARK_MITOTIC_SPINDLE_gsea_H.csv.gz	1.7 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Trt3.vs.Disease_HALLMARK_MYC_TARGETS_V1_gsea_H.csv.gz	3.0 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Trt3.vs.Disease_HALLMARK_OXIDATIVE_PHOSPHORYLATION_gsea_H.csv.gz	2.5 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Trt3.vs.Disease_HALLMARK_REACTIVE_OXYGEN_SPECIES_PATHWAY_gsea_H.csv.gz	834 b	(ftp)(http)	CSV
GSE233743_Exp3_Trt3.vs.Disease_deg_down_fdr_0.05_fc_1.5.csv.gz	103.2 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Trt3.vs.Disease_deg_up_fdr_0.05_fc_1.5.csv.gz	101.2 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Trt3.vs.Disease_gsea_H.csv.gz	5.1 Kb	(ftp)(http)	CSV
GSE233743_Exp3_Trt3.vs.Disease_top_hits.csv.gz	980.6 Kb	(ftp)(http)	CSV
GSE233743_Exp3_process_count_data_salmon.html.gz	3.9 Mb	(ftp)(http)	HTML
GSE233743_Exp3_sample_list.docx.gz	11.1 Kb	(ftp)(http)	DOCX
GSE233743_FC_comparison.xlsx	1.2 Mb	(ftp)(http)	XLSX
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Processed data are available on Series record

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