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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 26, 2023 |
| Title |
Mouse Thyroid Nanopore RNAseq |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In the present work, we pull attention to a remarkable phenomenon of a perpetually and robustly upregulated gene, the thyroglobulin gene (Tg). The gene is expressed in the thyroid gland and, as it has been recently demonstrated, forms so called transcription loop easily observable with light microscopy. Using this feature, we show that Tg is expressed at a high level from the moment a thyroid cell acquires its identity and both of the alleles remain highly active over the entire cell life, i.e. depending on a species, for months or years. We demonstrate that the high upregulation is characteristic of thyroglobulin genes in all major vertebrate groups. We prove that Tg is not influenced by the organismal levels of T3/T4 hormones, does not oscillate round the clock and is expressed during both exocrine and endocrine phases of the thyrocyte activity. We conclude that the thyroglobulin gene represents a valuable model to study maintenance of a high transcriptional upregulation.
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| Overall design |
We performed RNA sequencing to investigate intron retention in Tg transcripts. For this three thyroid glands were isolated and mRNAs were analyzed using Nanopore sequencing.
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| Contributor(s) |
Solovei I, Blum H, Krebs S, Ullrich S |
| Citation(s) |
37860816 |
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| Submission date |
May 25, 2023 |
| Last update date |
Oct 31, 2023 |
| Contact name |
Irina Solovei |
| E-mail(s) |
irina.solovei@lrz.uni-muenchen.de
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| Organization name |
LMU Munich
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| Street address |
Großhaderner Str. 2
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| City |
Planegg-Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA976298 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE233457_RAW.tar |
14.1 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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