|
| Status |
Public on Jan 01, 2024 |
| Title |
The transcriptome profiling of multi-cell types by the droplet based full-length single-cell transcript sequencing |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We developed a scalable Tn5-based Full-Length In situ Transcriptome sequencing method (scFLIT-seq). This method combines the advantages of in situ reverse transcription of methanol-fixed cells, single-cell separation and barcode ligation via the 10X scATAC-seq protocol and microfluidic platform, and Tn5-mediated fragmentation and tagging of cDNA. Various cells treated with different concentrations of methanol were used to validate the efficacy of this method. The efficacy at the single-cell level of this method was validated on a mixed cell population of HEK293T and NIH/3T3 cells.
|
| |
|
| Overall design |
The reverse transcription efficiency analysis of the FLIT-seq under different conditions in multi-cell types.
|
| |
|
| Contributor(s) |
Xu T, Xu Y, An Z, Xu J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| BioProject |
PRJNA973228 |
| |
| Submission date |
May 22, 2023 |
| Last update date |
Jan 01, 2024 |
| Contact name |
Yicong Xu |
| E-mail(s) |
maodatou88@hotmail.com
|
| Organization name |
Sun Yat-sen University
|
| Street address |
No. 135, Xingang Xi Road
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510275 |
| Country |
China |
| |
|
| Platforms (3) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL25526 |
Illumina NovaSeq 6000 (Homo sapiens; Mus musculus) |
|
| Samples (10)
|
|
Less...
More...