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| Status |
Public on Jun 27, 2024 |
| Title |
Discovery of an embryonically derived bipotent population of endothelial-macrophage progenitor cells in postnatal aorta |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Converging evidence indicates that extra-embryonic yolk sac is the source of both macrophages and endothelial cells in adult mouse tissues. Prevailing views are that these embryonically derived cells are maintained after birth by proliferative self-renewal in their differentiated states. Here we identify clonogenic endothelial-macrophage (EndoMac) progenitor cells in the adventitia of embryonic and postnatal mouse aorta, that are independent of Flt3-mediated bone marrow hematopoiesis and derive from an early embryonic CX3CR1+ and CSF1R+ source. These bipotent progenitors are proliferative and vasculogenic, contributing to adventitial neovascularization and forming perfused blood vessels after transfer into ischemic tissue. We establish a regulatory role for angiotensin II, which enhances their clonogenic and differentiation properties and rapidly stimulates their proliferative expansion in vivo. Our findings demonstrate that embryonically derived EndoMac progenitors participate in local vasculogenic responses in the aortic wall by contributing to the expansion of endothelial cells and macrophages postnatally.
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| Overall design |
Haematopoietic colony forming unit- macrophages cultured from freshly prepared cell suspecsions of postnatal mouse aorta were harvested and FACS-purified viable cells analysed using scRNA-seq.
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| Web link |
https://www.nature.com/articles/s41467-024-51637-7
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| Contributor(s) |
Williamson AE, Psaltis PJ |
| Citation(s) |
39154007 |
| |
| Submission date |
May 16, 2023 |
| Last update date |
Sep 26, 2024 |
| Contact name |
Peter James Psaltis |
| E-mail(s) |
peter.psaltis@sahmri.com
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| Phone |
+61 432359686
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| Organization name |
University of Adelaide
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| Department |
Adelaide Medical School
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| Street address |
North Terrace
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| City |
Adelaide |
| State/province |
South Australia |
| ZIP/Postal code |
5000 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
| GSM7360136 |
aorta, cultured CFU-M progenitors [Pool_1_GEX] |
| GSM7360137 |
MULTI-seq barcode library [Pool_2_MS] |
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| Relations |
| BioProject |
PRJNA973045 |
