|
| Status |
Public on Dec 12, 2024 |
| Title |
NKAPL binding sites in mouse testis and the genome-wide occupancy of the SOX30/HDAC3 protein complex and RNA Pol II in the absence of NKAPL |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To profile genomic binding sites of NKAPL and examine whether its knockout alters the genome-wide occupancy of the SOX30/HDAC3 protein complex and RNA Pol II in mouse testes, wild-type and Nkapl KO testes at postnatal day 21 were harvested for ChIP-seq.
|
| |
|
| Overall design |
Wild-type and Nkapl KO mouse testes were havested at postnatal day 21. ChIP experiments were performed with the indicated antibodies for NKAPL, SOX30, HDAC3, and RNA Pol II. Precipitated DNA was amplified according to ChIP Sequencing Sample Preparation Guide (Illumina).
|
| |
|
| Contributor(s) |
Kang Z, Xu C, Zhang J, Ye L |
| Citation(s) |
39824811 |
| |
| Submission date |
May 12, 2023 |
| Last update date |
Feb 18, 2025 |
| Contact name |
Chen Xu |
| E-mail(s) |
xuchendhr@outlook.com
|
| Phone |
15090769649
|
| Organization name |
Nanjing Medical University
|
| Street address |
101 Longmian Avenue, Jiangning District
|
| City |
Nanjing |
| ZIP/Postal code |
210000 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (20)
|
|
| Relations |
| BioProject |
PRJNA971928 |
Less...
More...