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| Status |
Public on Apr 04, 2024 |
| Title |
Single cell gene expression profile of tumor-associated fibroblasts from macrophage-depleted PDAC liver metastasis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used single cell RNA sequencing to investigate how macrophages regulate tumor-associated fibroblast heterogeneity in PDAC liver metastasis
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| Overall design |
Experimental liver metastasis in Pdgfrb-GFP reporter mouse model for 14 days. Macrophages were depleted with CSF1R neutralizing antibody, or IgG control, from Day 7 to 14. GFP+ metastasis-associated fibroblasts and GFP+ cells from naive liver were enriched by fluorescence activated cell sorting (FACS) and analyzed by single cell RNA sequencing
Contributor: Centre for Genomics Research, Liverpool
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| Contributor(s) |
Raymant M, Astuti Y, Green D, Schmid M |
| Citation(s) |
38678021 |
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| Submission date |
May 11, 2023 |
| Last update date |
May 24, 2024 |
| Contact name |
Michael C Schmid |
| E-mail(s) |
mschmid@liverpool.ac.uk
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| Organization name |
University of Liverpool
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| Department |
Molecular and Clinical Cancer Medicine
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| Lab |
Schmid Lab
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| Street address |
Ashton Street
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| City |
Liverpool |
| State/province |
Merseyside |
| ZIP/Postal code |
L69 3GE |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA971635 |
