 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 15, 2023 |
| Title |
MiR-431 attenuates synaptic plasticity and memory deficits in APPswe/PS1dE9 mice |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Synaptic plasticity impairment plays a critical role in the pathogenesis of Alzheimer’s disease (AD), and emerging evidence has shown that microRNAs (miRNAs) are alternative biomarkers and therapeutic targets for synaptic dysfunctions in AD. In this study, we found that the level of miR-431 was downregulated in the plasma of amnestic mild cognitive impairment (aMCI) and AD patients. In addition, it was decreased in the hippocampus and plasma of APPswe/PS1dE9 (APP/PS1) mice. Lentivirus mediated miR-431 overexpression in the hippocampus CA1 ameliorated synaptic plasticity and memory deficits of APP/PS1 mice, while it didn't affect the Aβ levels. Smad4 was identified as a target of miR-431, and Smad4 knockdown modulated the expression of synaptic proteins including SAP102, and protected against synaptic plasticity and memory dysfunctions in APP/PS1 mice. Furthermore, Smad4 overexpression reversed the protective effects of miR-431, indicating that miR-431 attenuated synaptic impairment at least partially by Smad4 inhibition. Thus, these results indicated that miR-431/Smad4 might be a potential therapeutic target for AD treatment.
|
| |
|
| Overall design |
Smad4 is a transcription factor,we did a cut&tag assay to find the target of smad4.
|
| |
|
| Contributor(s) |
Ge J, Xue Z, Shu S, Zhu X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 08, 2023 |
| Last update date |
May 17, 2023 |
| Contact name |
jianwei ge |
| E-mail(s) |
gejw410@sina.com
|
| Phone |
17826866117
|
| Organization name |
nanjing university
|
| Street address |
zhongshan road
|
| City |
nanjing |
| ZIP/Postal code |
210000 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA970361 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE231985_RAW.tar |
4.2 Mb |
(http)(custom) |
TAR (of BW, NARROWPEAK) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
