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| Status |
Public on May 09, 2023 |
| Title |
PC3T: a signature-driven predictor of chemical compounds for cellular transition |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cellular transitions hold great promise in translational medicine research. However, therapeutic applications are limited by the low efficiency and safety concerns of using transcription factors. Small molecules provide a temporal and highly tuneable approach to overcome these issues. Here, we present PC3T, a computational framework to identify enrich molecules that induce desired cellular transitions, and PC3T was able to consistently identify enrich small molecules that had been experimentally validated in both bulk and single cell datasets. We then predicted and experimentally validated small molecules reprogramming of fibroblasts into hepatocyte-like cells and found that the fibroblasts exhibited epithelial cell morphology and hepatocyte-like specific gene expression pattern after treatment.
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| Overall design |
Examine RNA expression profile of MEFs, and MEFs treated by 3 molecules: Carbidopa, CHIR-99021 and LY-364747.
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| Contributor(s) |
Cui X, Han L, Zhou W, Zhang P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 08, 2023 |
| Last update date |
May 09, 2023 |
| Contact name |
Xiuliang Cui |
| E-mail(s) |
cuixl_bio@126.com
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| Phone |
18321673089
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| Organization name |
Second Military Medicine University
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| Street address |
Changhai Road 225
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200438 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA970319 |
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