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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 29, 2024 |
| Title |
Respiratory Syncytial virus (RSV) directly binds host microRNAs to de-regulate host gene targets [RNA-seq miR-27 over-expression] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To validate miR-27 mRNA targets, we generated miR-27 overexpressing cell lines using the shMIMIC lentiviral transduction system (Dharmacon)
In addition, we transfected miR-27 miRNA mimics and inhibitors to overexpress and inhibit the miRNA and performed total RNAseq
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| Overall design |
RNA-seq data of A549 cells, WT and miR-27 overexpressing (shMIMIC) lines, as well as mimic and inhibitor transfected cells
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| Contributor(s) |
Ressel S, Bermudez-Barrientos JR, Kumar S, Gordon K, Lane J, Wu J, Abreu-Goodger C, Buck A |
| Citation(s) |
38412296 |
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| Submission date |
May 05, 2023 |
| Last update date |
May 29, 2024 |
| Contact name |
José Roberto Bermúdez-Barrientos |
| E-mail(s) |
roberto89bermudez@gmail.com, beto.bermudez@ed.ac.uk, sarah.ressel@ed.ac.uk
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| Organization name |
The University of Edinburgh
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| Department |
Institute of Immunology & Infection
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| Lab |
Buck Lab
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| Street address |
Charlotte Auerbach Rd
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| City |
Edinburgh |
| State/province |
Lothian |
| ZIP/Postal code |
EH9 3AZ |
| Country |
United Kingdom |
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| Platforms (1) |
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| Samples (19)
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| GSM7300391 |
A549 miR-27a shMIMIC clone 1, Mock-infected,rep 1 |
| GSM7300392 |
A549 non-targeting control (NTC) shMIMIC clone 1, Mock-infected rep 1 |
| GSM7300393 |
A549 WT, Mock-infected rep 1 |
| GSM7300394 |
A549 miR-27a shMIMIC clone 1, Mock-infected,rep 2 |
| GSM7300395 |
A549 NTC shMIMIC clone 1, Mock-infected rep 2 |
| GSM7300396 |
A549 WT, Mock-infected rep 2 |
| GSM7300397 |
A549 miR-27a shMIMIC clone 1, Mock-infected,rep 3 |
| GSM7300398 |
A549 NTC shMIMIC clone 1, Mock-infected rep 3 |
| GSM7300399 |
A549 WT, Mock-infected rep 3 |
| GSM7300400 |
A549 miR-27a & miR-27b mimic transfected, Mock-infected,rep 1 |
| GSM7300401 |
A549 miR-27a & miR-27b inhibitor transfected, Mock-infected,rep 1 |
| GSM7300402 |
A549 control mimic transfected, Mock-infected,rep 1 |
| GSM7300403 |
A549 control inhibitor transfected, Mock-infected,rep 1 |
| GSM7300404 |
A549 miR-27a & miR-27b mimic transfected, Mock-infected,rep 2 |
| GSM7300405 |
A549 miR-27a & miR-27b inhibitor transfected, Mock-infected,rep 2 |
| GSM7300406 |
A549 control mimic transfected, Mock-infected,rep 2 |
| GSM7300407 |
A549 control inhibitor transfected, Mock-infected,rep 2 |
| GSM7300408 |
A549 miR-27a & miR-27b mimic transfected, Mock-infected,rep 3 |
| GSM7300409 |
A549 miR-27a & miR-27b inhibitor transfected, Mock-infected,rep 3 |
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA967684 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE231787_SR106_genes_n_regRegions_raw_counts_for_degust_GEO.csv.gz |
3.8 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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