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| Status |
Public on Apr 15, 2024 |
| Title |
Impact of azide metabolic glycoengineering and bioorthogonal click chemistry cell-cell tethering in adipose tissue-derived mesenchymal stromal cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report RNA sequencing data from adipose tissue-derived mesenchymal stromal cells (hASCs) functionalized via metabolic glycoengineering with pendant azide sialosides. Beyond the impact of this cell surface engineering on transcriptome, we explore the expression profiles of azide-hASCs covalently tethered to an interfacial glycosaminoglycan via bioorthogonal click chemistry and yielding a 3D multicellular construct. We find global gene expression changes in response to metabolic glycoengineering in 2D cell culture conditions as well as those arising from cell-to-cell tethering into 3D environments.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for hASCs in three conditions (2D Native, 2D Glycoengineered and 3D Day 1 Cellgels)
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| Contributor(s) |
Lavrador P, Moura BS, Almeida-Pinto J, Gaspar VM, Mano JF |
| Citation missing |
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| BioProject |
PRJNA949992 |
| |
| Submission date |
May 02, 2023 |
| Last update date |
Apr 15, 2024 |
| Contact name |
Pedro Lavrador |
| E-mail(s) |
pedrolavrador@ua.pt
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| Organization name |
University of Aveiro
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| Department |
CICECO - Aveiro Institute of Materials
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| Street address |
Campus Universitário de Santiago, Universidade de Aveiro
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| City |
Aveiro |
| ZIP/Postal code |
3810-193 |
| Country |
Portugal |
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| Platforms (1) |
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| Samples (15)
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