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| Status |
Public on Apr 26, 2023 |
| Title |
Sex differences in the effects of caloric restriction (CR) on hepatic gene expression in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The effects of caloric restriction (CR) on glucose homeostasis and metabolic function differ between males and females. To investigate if hepatic function contributes to these sex differences, we collected liver samples from male and female C57BL/6NCrl mice that were fed ad libitum (AL) or underwent caloric restriction (CR; receiving 70% of daily AL intake) from 9-13 weeks of age. Livers were then analysed by bulk RNA-seq.
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| Overall design |
The goal of this study was to determine how caloric restriction (CR) influences the hepatic transcriptome and if this differs between males and females. The experiment included five ad libitum (AL)-fed females, six CR females, six AL males and five CR males. Mice were fed AL or CR from 9-13 weeks of age. To minimise effects resulting from differences in fasting duration, mice in both the AL and CR groups were fasted for ~8 hours prior to necropsy and liver sampling.
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| Contributor(s) |
Cawthorn WP, Ikushima YM, Kobayashi H, Chen K |
| Citation(s) |
37096321 |
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https://doi.org/10.7554/eLife.88080
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| Submission date |
Apr 24, 2023 |
| Last update date |
Apr 26, 2023 |
| Contact name |
William P Cawthorn |
| Organization name |
The University of Edinburgh
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| Department |
University/BHF Centre for Cardiovascular Science
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| Lab |
Cawthorn lab
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| Street address |
47 Little France Crescent
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| City |
Edinburgh |
| ZIP/Postal code |
EH16 4TJ |
| Country |
United Kingdom |
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| Platforms (1) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA961073 |
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