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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 03, 2023 |
| Title |
A2AR-eGFP reporter mouse enables elucidation of A2AR expression dynamics during anti-tumor immune responses |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Understanding of the mechanism by which A2AR is regulated has been limited by difficulty in identifying the cell types that express A2AR due to a lack of robust antibodies for these receptors. To overcome this we developed an A2AR-eGFP reporter mouse and assessed the expression of A2AR during ongoing anti-tumor immune responses.
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| Overall design |
A2AR eGFP reporter mice were injected with 5 x 10^5 AT-3 ova tumors subcutaneously and where indicated treated at days 14 and 18 post tumor inoculation with anti-PD-L1. At day 21 post tumor inoculation CD8+ GFP+ or GFP- tumor-infiltrating lymphocytes were flow sorted and analyzed by 3’RNA-Sequencing.
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| Contributor(s) |
Todd K, Beavis P, Sek K |
| Citation(s) |
37914685 |
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| Submission date |
Apr 20, 2023 |
| Last update date |
Nov 16, 2023 |
| Contact name |
Kevin C sek |
| E-mail(s) |
kevin.sek@petermac.org
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| Phone |
0432960331
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| Organization name |
Peter MacCallum Cancer Centre
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| Department |
Cancer Immunology
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| Lab |
Beavis Lab
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| Street address |
305 Grattan Street
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| City |
Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
3000 |
| Country |
Australia |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA957714 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE230135_Counts_table.tsv.gz |
583.8 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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