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Series GSE229916 Query DataSets for GSE229916
Status Public on May 10, 2024
Title Pericytes and perivascular fibroblasts contribute to central nervous system fibrosis in a region dependent manner
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Fibrotic scar tissue formation is conserved throughout the central nervous system in humans and mice, and impairs tissue regeneration and functional recovery. However, the origin of scar-forming stromal fibroblasts is controversial. Here, we show that stromal fibroblasts found after spinal cord injury derive from two populations of perivascular cells that are anatomically and transcriptionally defined as pericytes and perivascular fibroblasts. We identify two distinct perivascular cell populations, which activate and transcriptionally converge on the generation of stromal myofibroblasts after injury. Our results suggest potential targets to improve tissue regeneration and functional recovery after central nervous system injuries.
 
Overall design We crossed GLAST-CreERT2;R26R-tdTomato mice to the Pdgfrb-eGFP reporter line, which drives EGFP expression in all PDGFRb+ perivascular cells (pericytes, vSMCs and fibroblasts). We isolated GLAST-expressing perivascular cells from the uninjured spinal cord and 5 days after spinal cord injury by fluorescence activated cell sorting (FACS) based on tdTomato and EGFP expression. To define the molecular identity and compare GLAST-expressing perivascular cells (tdTomato+EGFP+) to other perivascular cells, we also sorted dTomato-EGFP+ cells. Sorted cells were subjected single cell sequencing using the SmartSeq 2 protocol.
 
Contributor(s) Holl D, Göritz C
Citation(s) 38849523
Submission date Apr 17, 2023
Last update date Aug 08, 2024
Contact name Christian Göritz
E-mail(s) christian.goeritz@ki.se
Organization name Karolinska Institutet
Department CMB
Street address Solnavägen 9
City Solna
ZIP/Postal code 17165
Country Sweden
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (832)
GSM7180389 Spinal cord, Uninjured, P6981_1001
GSM7180390 Spinal cord, Uninjured, P6981_1002
GSM7180391 Spinal cord, Uninjured, P6981_1003
Relations
BioProject PRJNA956577

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE229916_170706_sampleinfo_scs.csv.gz 10.5 Kb (ftp)(http) CSV
GSE229916_rpkmforgenes_counts.tab.txt.gz 5.5 Mb (ftp)(http) TXT
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