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| Status |
Public on May 15, 2023 |
| Title |
Effect of Interferon Gamma and Hydroxyproline on Gene Expression in THP-1 Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Hydroxyproline (trans-4-Hydroxy-L-proline) is an abundant amino acid in mammalian tissue, yet little is known about how its prescence effects gene expression. This study aimed to analyze the effects of hydroxyproline on gene expression in THP-1 cells stimulated with IFN-γ, using RNA-seq.
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| Overall design |
RNA-seq analysis was perfromed on samples from THP-1 cells treated for 72 hours with a vehicle control alone, vehicle and IFN-γ (50 ng/ml), or hydroxyproline (40 mM) and IFN-γ were compared. Three replicates per condition were included. Differential gene expression analysis was performed.
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| Contributor(s) |
Spangenberg SH, Palermo A, Lairson LL, Siuzdak G |
| Citation(s) |
37467751 |
| |
| Submission date |
Apr 14, 2023 |
| Last update date |
Oct 10, 2024 |
| Contact name |
Stephan Spangenberg |
| E-mail(s) |
stephan.wcb@gmail.com
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| Phone |
9253255007
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| Organization name |
The Scripps Research Institute
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| Department |
Chemistry
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| Lab |
Lairson
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| Street address |
10550 N Torrey Pines Rd, La Jolla, CA 92037
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| City |
SAN DIEGO |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
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| Platforms (1) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA955675 |
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