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Series GSE22934 Query DataSets for GSE22934
Status Public on Apr 08, 2011
Title Wdr5 mediates self-renewal and reprogramming via the embryonic stem cell core transcriptional network
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The embryonic stem (ES) cell transcriptional and epigenetic networks are critical for the maintenance of ES cell self-renewal. However, it remains unclear whether components of these networks functionally interact and if so, what factors mediate such interactions. Here we show that WD-repeat protein-5 (Wdr5), a core member of the mammalian Trithorax (trxG) complex, positively correlates with the undifferentiated state and is a novel regulator of ES cell self-renewal. We demonstrate that Wdr5, an ‘effector’ of H3K4 methylation, interacts with the pluripotency transcription factor Oct4. In concordance, genome-wide ChIP-Seq and transcriptome analyses demonstrate overlapping gene regulatory functions between Oct4 and Wdr5. We show that the Oct4-Sox2-Nanog circuitry cooperates with trxG for transcriptional activation of key self-renewal regulators. Furthermore, Wdr5 expression is required for the efficient formation of induced pluripotent stem (iPS) cells. Collectively, these findings implicate an integrated model of transcriptional and epigenetic control, mediated by select trxG members, for the maintenance of ES cell self-renewal and somatic cell reprogramming.
 
Overall design 7 Samples
 
Contributor(s) Ang Y, Tsai S, Lee D, Monk J, Su J, Ratnakumar K, Ge Y, Darr H, Chang B, Rendl M, Bernstein E, Schaniel C, Lemischka IR
Citation(s) 21477851
Submission date Jul 14, 2010
Last update date May 15, 2019
Contact name Jonathan Monk
E-mail(s) jonathan.m.monk@gmail.com
Phone 9716457785
Organization name Mt. Sinai School of Medicine
Department Gene and Cell Medicine
Lab Lemischka
Street address One Gustave L. Levy Place
City New York
State/province NY
ZIP/Postal code 10029
Country USA
 
Platforms (1)
GPL9185 Illumina Genome Analyzer (Mus musculus)
Samples (7)
GSM566276 H3K4me3_ChIP-seq
GSM566277 Oct4_ChIP-seq
GSM566278 Rbbp5_ChIP-seq
Relations
SRA SRP002862
BioProject PRJNA127937

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22934_RAW.tar 6.5 Gb (http)(custom) TAR (of BED, TXT, WIG)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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