NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE229022 Query DataSets for GSE229022
Status Public on Mar 21, 2024
Title Single nuclei gene expression profile for adult C. elegans and several longevity mutants
Organism Caenorhabditis elegans
Experiment type Expression profiling by high throughput sequencing
Summary We presents a complete single-cell atlas of aging worms, encompassing both somatic and germ cell types. This atlas was built upon 241K nuclei across the worm lifespan at day 1, 6, 12, and 14 (100%, 99%, 61%, and 14% survival rates, respectively). The worms were aged under normal physiological conditions, without interfering with their reproductive processes.
 
Overall design To perfom snRNAseq for wild-type and longevity worms, we harvested and homogenized ~2,000 worms for each genotype/condition. Nuclei were isolated using fluorescence-activated cell sorting (FACS) based on the DNA content signal and then proceed with 10x Chromium sequcing.
Web link https://pubmed.ncbi.nlm.nih.gov/38816550/
 
Contributor(s) Gao SM, Qi Y, Li H, Fu Y, Wang M
Citation(s) 38816550
Submission date Apr 05, 2023
Last update date Jun 20, 2024
Contact name Shihong Max Gao
E-mail(s) gaos2@hhmi.org
Organization name Janelia Research Campus
Department 4DCP
Lab Meng Wang
Street address 19700 Helix Dr
City Ashburn
ZIP/Postal code 20147
Country USA
 
Platforms (3)
GPL26672 Illumina NovaSeq 6000 (Caenorhabditis elegans)
GPL27998 NextSeq 550 (Caenorhabditis elegans)
GPL32326 NextSeq 2000 (Caenorhabditis elegans)
Samples (28)
GSM7147945 Day1 Wild-type(N2) feed with E. coli K-12 BW25113
GSM7147946 Day6 Wild-type(N2) feed with E. coli K-12 BW25113
GSM7147947 Day1 daf-2 mutant feed with E. coli OP50
Relations
BioProject PRJNA952585

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE229022_RAW.tar 904.1 Mb (http)(custom) TAR (of MTX, TSV)
GSE229022_integrated_seurat_revision.rds.gz 7.7 Gb (ftp)(http) RDS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap