We conducted a comprehensive evaluation of the updated Infinium MethylationEPIC v2 BeadChip (EPICv2). Our evaluation revealed that EPICv2 offers significant improvements over its predecessors, including expanded enhancer coverage, applicability to diverse ancestry groups, support for low-input DNA down to one nanogram, coverage of existing epigenetic clocks, cell type deconvolution panels, and human trait associations, while maintaining accuracy and reproducibility.
Overall design
Cell line (GM12878, K562, LNCaP, HCT116 and derivative cell lines) DNA and methylation control DNA is run on EPIC and/or EPICv2 BeadChips to evaluate EPICv2 reproducibility, accuracy, performance at low input ranges, and capability at capturing biological methylation variation.
Please note that *control* samples are DNA ordered from EpigenDx. The exact cell line source is unknown (i.e. mixed cell line-derived DNA).
The study is published at Comprehensive evaluation of the Infinium human MethylationEPIC v2 BeadChip D Kaur, SM Lee, D Goldberg, NJ Spix, T Hinoue, HT Li, VB Dwaraka, ... Epigenetics Communications 3 (1), 6
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