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| Status |
Public on Apr 06, 2023 |
| Title |
Human motor neurons are rare in the spinal cord and can be transcriptomically divided into known subtypes divided into known subtypes. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Motor neurons are a rare neuronal subtype in the adult spinal cord. We performed single-nucleus RNA sequencing on nuclei extracted from adult human spinal cord and describe the transcriptional heterogeneity.
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| Overall design |
Nuclei from the cervical spinal cord of a neurologically healthy control patient were isolated, homogenized, and stained with antibodies against neuronal marker NeuN. Nuclei were sorted by FANS and transcriptomes captured using 10x V3.1 3' kits.
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| Contributor(s) |
Gautier O, Blum J, Maksymetz J, Chen D, Schweingruber C, Mei I, Hermann A, Hackos D, Hedlund E, Ravits J, Gitler A |
| Citation missing |
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| Submission date |
Apr 03, 2023 |
| Last update date |
Apr 09, 2023 |
| Contact name |
Jacob Blum |
| E-mail(s) |
jacobablum@gmail.com
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| Organization name |
Stanford University
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| Street address |
279 Campus Dr
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| City |
Stanford |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA951608 |
