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Series GSE228778 Query DataSets for GSE228778
Status Public on Apr 06, 2023
Title Human motor neurons are rare in the spinal cord and can be transcriptomically divided into known subtypes divided into known subtypes.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Motor neurons are a rare neuronal subtype in the adult spinal cord. We performed single-nucleus RNA sequencing on nuclei extracted from adult human spinal cord and describe the transcriptional heterogeneity.
Overall design Nuclei from the cervical spinal cord of a neurologically healthy control patient were isolated, homogenized, and stained with antibodies against neuronal marker NeuN. Nuclei were sorted by FANS and transcriptomes captured using 10x V3.1 3' kits.
Contributor(s) Gautier O, Blum J, Maksymetz J, Chen D, Schweingruber C, Mei I, Hermann A, Hackos D, Hedlund E, Ravits J, Gitler A
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Submission date Apr 03, 2023
Last update date Apr 09, 2023
Contact name Jacob Blum
Organization name Stanford University
Street address 279 Campus Dr
City Stanford
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (2)
GSM7136257 S1_Cerv_131 biol rep 1
GSM7136258 S2_Cerv_131 biol rep 2
BioProject PRJNA951608

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE228778_RAW.tar 370.9 Mb (http)(custom) TAR (of H5)
GSE228778_gautier_mns.rds.gz 9.3 Mb (ftp)(http) RDS
GSE228778_gautier_neurons.rds.gz 3.9 Gb (ftp)(http) RDS
GSE228778_gautier_prop_intronic.rds.gz 5.9 Gb (ftp)(http) RDS
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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