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Series GSE228696 Query DataSets for GSE228696
Status Public on Apr 19, 2023
Title Short-term effects of overexpression of GK and/or GKRP on the transcriptome in mouse primary hepatocytes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Short-term effects of overexpression of GK and/or GKRP on the transcriptome in mouse primary hepatocytes
 
Overall design GKRP-deficient hepatocytes from female GCKR-P446L-EMI-B6N mice (n=3: LL; LL; LP) were cultured in monolayer and treated with adenoviral vectors for GK or GKRP(446P or 446L) expression
Sequences for GKRP vectors were Human: BC130481; Mouse: XM_006503883; GK PMID: 8550593.
After 24h-culture in MEM with 5mM glucose, they were incubated for 4h in medium containing 25mM glucose and then harvested for RNA extraction and sequencing
Web link https://doi.org/10.1016/j.molmet.2023.101722
 
Contributor(s) Agius L, Ford BE
Citation(s) 37031802
Submission date Apr 01, 2023
Last update date Apr 23, 2023
Contact name Loranne Agius
E-mail(s) loranne.agius@newcastle.ac.uk
Phone +441912087033
Organization name Newcastle University
Department Biosciences Institute
Lab Glucokinase Lab
Street address Framlington Place
City Newcastle upon Tyne
ZIP/Postal code NE2 4HH
Country United Kingdom
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (36)
GSM7135047 UN1, hepatocyte-1 untreated
GSM7135048 UN2, hepatocyte-1 untreated
GSM7135049 HP1, hepatocyte-1 human GKRP-446P expression
Relations
BioProject PRJNA951223

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE228696_RAW.tar 56.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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