|
| Status |
Public on Jan 01, 2024 |
| Title |
Single-cell RNA-sequencing of tumor-infiltrating cells from intra-tibial injection of LLC1 cells in murine model. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We injected LLC1 cells into tibia to construct a murine model to study bone microenvironment in cancer bone metastasis. We used single cell RNA sequencing (scRNA-seq) to analyze the cellular heterogeneity of tumor-infiltrating cells.
|
| |
|
| Overall design |
LLC1 cells were injected intra-tibially into CAG-ZsGreen mice. 14 days later tumor-bearing tibia were dissected and enzymatically dissociated into single cell suspension. Tumor-infiltrating cells were isolated by Fluorescence-activated cell sorting (FACS) according to the presence of ZsGreen signal and analyzed using scRNAseq.
|
| |
|
| Contributor(s) |
Zhou H, Zhang W, Gao J, Ye Z |
| Citation(s) |
38232158 |
| |
| Submission date |
Mar 30, 2023 |
| Last update date |
Jan 24, 2024 |
| Contact name |
Zhaoming Ye |
| E-mail(s) |
yezhaoming@zju.edu.cn
|
| Organization name |
The Second Affiliated Hospital, Zhejiang University School of Medicine
|
| Street address |
88 jiefang road
|
| City |
hangzhou |
| ZIP/Postal code |
310031 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (1) |
| GSM7133941 |
tumor-infiltrating cell in intratibial LLC model |
|
| Relations |
| BioProject |
PRJNA950433 |
