|
| Status |
Public on Apr 28, 2023 |
| Title |
CIRCLE-seq analyais for -175 site |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Adenosine base editing to recreate the HPFH mutations is a promising approach to induce HbF. We used CIRCLE-seq to identify 682 potential off-target sites in purified DNA treated with Cas9 nuclease complexed to sgRNA targeting -globin -175 .
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| |
|
| Overall design |
CIRCLE-seq was performed in HEK293T cells.
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| |
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| Contributor(s) |
Mayuranathan T, Yen J |
| Citation(s) |
37400614 |
| |
| Submission date |
Mar 30, 2023 |
| Last update date |
Jan 02, 2024 |
| Contact name |
Jonanthan Yen |
| E-mail(s) |
thiyagarajibms@gmail.com
|
| Organization name |
St. Jude Children's Research Hospital
|
| Department |
Hematology
|
| Street address |
262 Danny Thomas Pl
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE228822 |
Potent and uniform fetal hemoglobin induction via base editing |
|
| Relations |
| BioProject |
PRJNA951689 |
